Detailed information of TA system TA141560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3393924..3394570
• Replicon: chromosome
• Accession: NZ_CP045257
• Organism: Proteus mirabilis strain L90-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8E4FP08
• Locus tag: GCE56_RS16470
• Protein ID: WP_060555121.1
• Coordinates: 3393924..3394271 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8E4FP64
• Locus tag: GCE56_RS16475
• Protein ID: WP_060555120.1
• Coordinates: 3394271..3394570 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCE56_RS16435	3389028..3389321	-	294	WP_049218683.1	YqjK-like family protein	-
GCE56_RS16440	3389318..3389679	-	362	Protein_3116	phage holin family protein	-
GCE56_RS16445	3389999..3391198	-	1200	WP_049218680.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
GCE56_RS16450	3391500..3391901	+	402	WP_049218678.1	PPC domain-containing protein	-
GCE56_RS16455	3392318..3392719	-	402	WP_060555124.1	phage holin family protein	-
GCE56_RS16460	3392801..3393154	-	354	WP_060555123.1	hypothetical protein	-
GCE56_RS16465	3393226..3393474	-	249	WP_060555122.1	DUF883 family protein	-
GCE56_RS16470	3393924..3394271	+	348	WP_060555121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCE56_RS16475	3394271..3394570	+	300	WP_060555120.1	XRE family transcriptional regulator	Antitoxin
GCE56_RS16480	3394691..3394879	+	189	WP_060555126.1	DUF5397 family protein	-
GCE56_RS16485	3394883..3395161	+	279	WP_060555119.1	virulence factor	-
GCE56_RS16490	3395634..3397124	+	1491	WP_060555118.1	YadA-like family protein	-
GCE56_RS16495	3397311..3397385	-	75	Protein_3127	IS200/IS605 family transposase	-
GCE56_RS16500	3397379..3397486	-	108	Protein_3128	transposase	-
GCE56_RS16505	3397536..3397742	-	207	WP_060555117.1	hypothetical protein	-
GCE56_RS16510	3397739..3398323	-	585	WP_114141483.1	AAA family ATPase	-
GCE56_RS16515	3398830..3399348	-	519	WP_060555115.1	ClbS/DfsB family four-helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J)	-	3264743..3440274	175531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13561.55 Da
• Isoelectric Point: 8.8337

>T141560 WP_060555121.1 NZ_CP045257:3393924-3394271 [Proteus mirabilis]
MWAVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRLYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 348 bp

>T141560 NZ_CP060015:c829322-829005 [Mycobacterium malmoense]
ATATTCATCGTTTCGCTTGACCCGACACAGGGTCATGAGCAGCGCGGCACACGCCCCGTGTTGATCGTGTCCCCGACCGC
CTTTAACCGTCTAACGCAAACTCCGGTGGTACTGCCAATAACGCGTGGCGGAAACTTCGCCCGCACAGCAGGTTTCGCGG
TGTCGCTAACAGATGCCGGCACCCGCACCACCGGCGTCATCCGCTGCGATCAACCCCGAGCAATCGATATCGGGTCACGC
AACGGGCGCAAAGTTGAACGCGTTCCGGCCGCGATTCTCGATGAAGTACTGGCCAAACTTGCCACGCTCTTCAGCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11142.01 Da
• Isoelectric Point: 5.8962

>AT141560 WP_060555120.1 NZ_CP045257:3394271-3394570 [Proteus mirabilis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNELKLATLKRYIEAMG
GKLSLDVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT141560 NZ_CP060015:c829570-829337 [Mycobacterium malmoense]
GTGGGGGGTTCGGTCATGCTGGCGGTCCCGCCCGCGGTGCTCGACATGCTGCGGCTGCACGCCGGGTCGGCGGTTGGCCT
GTCGGTCGATGCCGGTCACTTGATAGTGGAACCGCAGACGCGTCCGCGTTACACCCTCGACGAGCTCTTGTCACAGTGCG
ACGCCTCAGCCGACCTCACGGATGAGGATAGGGTTTGGCTCGATGGCGAACCCGTCGGCGGTGAGTTGCTCTGA