Detailed information of TA system TA141520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3544243..3544897
• Replicon: chromosome
• Accession: NZ_CP045221
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain ARS 38

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FD951_RS15855
• Protein ID: WP_023964380.1
• Coordinates: 3544243..3544593 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FD951_RS15860
• Protein ID: WP_023964378.1
• Coordinates: 3544583..3544897 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD951_RS15845	3540992..3542524	+	1533	WP_023964383.1	NADH-quinone oxidoreductase subunit M	-
FD951_RS15850	3542532..3543995	+	1464	WP_009044594.1	NADH-quinone oxidoreductase subunit NuoN	-
FD951_RS15855	3544243..3544593	+	351	WP_023964380.1	toxin	Toxin
FD951_RS15860	3544583..3544897	+	315	WP_023964378.1	transcriptional regulator	Antitoxin
FD951_RS15865	3545077..3546819	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
FD951_RS15870	3546870..3547142	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
FD951_RS15875	3547153..3547953	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
FD951_RS15880	3547965..3548831	-	867	WP_023964372.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13674.61 Da
• Isoelectric Point: 9.4187

>T141520 WP_023964380.1 NZ_CP045221:3544243-3544593 [Pseudomonas chlororaphis subsp. aurantiaca]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTGGLRKIRFVDERRHKGKRGGIRVIYYGWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T141520 NZ_CP059997:2731476-2731736 [Amycolatopsis sp. CA-230715]
ATGAAGCTCGTCTGGGACGCGTTCGCCTGGGACGACTACCTCTGGCGGCAAACCCAGGATCGCAAGGTCCTCAAGCGGAT
CAACGACCTCCTGAAGGACGTCCGGCGCAACGGGAACGAAGGCATCGGCAAGCCCGAGCCGCTGAAACACGGTTTCCAGG
GCTACTGGTCCCGTCGGATCACTGACGAGCACCGACTGGTCTACAAGATCGAAGGCGACGAAATTCGCATCGCGGCTTGC
CGATACCACTACGGCAAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11861.78 Da
• Isoelectric Point: 10.7886

>AT141520 WP_023964378.1 NZ_CP045221:3544583-3544897 [Pseudomonas chlororaphis subsp. aurantiaca]
MKRDIFSELVEGFDALAKERQGKLTLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNARTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT141520 NZ_CP059997:2731237-2731479 [Amycolatopsis sp. CA-230715]
ATGAGCTACTCGGAGTCCCGCGCCAACTACGCGGAGACTCTTGACTCGGTGGTGAATGACCGCGAGGAAGTAGTGATCAC
CCGCGCTGGTCACGACCCCGTGGTGATCGTGTCGCTCGACGACTACGAATCACTGAAGGAAACGGCGTACCTGCTCCGCA
GCCCGGAGAACGCCCGGCGTCTCGTCTCCGCCATCGAACGGCTGGAGTCGGGTGGCGGCGAGCAGCACGAACTCGCCGAA
TGA