Detailed information of TA system TA141514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2111700..2112354
• Replicon: chromosome
• Accession: NZ_CP045216
• Organism: Pantoea dispersa strain BJQ0007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GAY20_RS09900
• Protein ID: WP_021508927.1
• Coordinates: 2111983..2112354 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NDM6
• Locus tag: GAY20_RS09895
• Protein ID: WP_021508926.1
• Coordinates: 2111700..2111999 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAY20_RS09865	2106862..2107620	+	759	WP_010615680.1	thiazole synthase	-
GAY20_RS09870	2107631..2108599	+	969	WP_021508921.1	HesA/MoeB/ThiF family protein	-
GAY20_RS09875	2108596..2109312	+	717	WP_152390908.1	ATP-binding cassette domain-containing protein	-
GAY20_RS09880	2109296..2110030	+	735	WP_058776076.1	ABC transporter permease	-
GAY20_RS09885	2110040..2110969	+	930	WP_152390909.1	ABC transporter substrate-binding protein	-
GAY20_RS09890	2111155..2111622	+	468	WP_058759022.1	hypothetical protein	-
GAY20_RS09895	2111700..2111999	-	300	WP_021508926.1	XRE family transcriptional regulator	Antitoxin
GAY20_RS09900	2111983..2112354	-	372	WP_021508927.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GAY20_RS09905	2112849..2113766	-	918	WP_021508930.1	LysR family transcriptional regulator	-
GAY20_RS09910	2113903..2114904	+	1002	WP_152390910.1	zinc-binding alcohol dehydrogenase family protein	-
GAY20_RS09915	2115009..2115662	+	654	WP_058770319.1	oxygen-insensitive NAD(P)H nitroreductase	-
GAY20_RS09920	2115692..2117071	-	1380	WP_104187545.1	heavy metal sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (10-88)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14423.65 Da
• Isoelectric Point: 10.3873

>T141514 WP_021508927.1 NZ_CP045216:c2112354-2111983 [Pantoea dispersa]
VWQVEATDRFWKWLQAQDEALRLDVLAAMKLLAQYGPHLGRPFVDTLMLSRVPNMKELRVQSKGRPIRSFFVFDPRRHAI
VLCAGNKQGKNQKRFYQQMLRIAETEYHHHLNAIGETHNENLG

Nucleotide

• Length: 372 bp

>T141514 NZ_CP059995:c5622685-5622503 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11265.00 Da
• Isoelectric Point: 6.7255

>AT141514 WP_021508926.1 NZ_CP045216:c2111999-2111700 [Pantoea dispersa]
MKTLDEMLAALTPEQQHQVDERVSQMRLEYQLYQLREQMQRTQRQQAQAMHIAQSSVAAMEARGAELKISTLKRYIEALG
GELTLGVKMPGGQHVDLII

Nucleotide

• Length: 300 bp

>AT141514 NZ_CP059995:c5622475-5622071 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Structures

Antitoxin

• AlphaFold DB: A0A246NDM6