Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4399649..4400448 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag GC231_RS21530 Protein ID WP_000347266.1
Coordinates 4399984..4400448 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GC231_RS21525 Protein ID WP_001307405.1
Coordinates 4399649..4399984 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS21510 (4395434) 4395434..4396204 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GC231_RS21515 (4396220) 4396220..4397554 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GC231_RS21520 (4397929) 4397929..4399500 + 1572 WP_001273741.1 galactarate dehydratase -
GC231_RS21525 (4399649) 4399649..4399984 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GC231_RS21530 (4399984) 4399984..4400448 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GC231_RS21535 (4400503) 4400503..4401312 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GC231_RS21540 (4401561) 4401561..4402841 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
GC231_RS21545 (4402864) 4402864..4403337 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GC231_RS21550 (4403348) 4403348..4404127 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
GC231_RS21555 (4404117) 4404117..4404995 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
GC231_RS21560 (4405013) 4405013..4405447 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4387382..4400448 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T141507 WP_000347266.1 NZ_CP045213:4399984-4400448 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T141507 NZ_CP059993:56106-56507 [Xanthomonas citri]
ATGAAGCGGTACATGCTTGATACCAACACCGTGAGCCATCTCGTCAAGAGCCATCCGGCTGTCTCTCGACGGGTCATTGA
AGTGCCTATGACTGCGCTATGTATGTCCGCAATTACCGGTGGGGAGCTGATGTTCGGCTTGGCTAAGGTGCCAGACGCCA
AACGCCTTCAGCAAGCCGTCATGGAACTCCTGCGGCGCGTGGATGTGCTGCCTTGGGATGGCGCGGTAATGGAGCGTTAC
GGAAGCGTAAGAGCGGACCTAGAAAAGCAAGGTAAAGCGCTTGGGTCGCTCGACATGCTTATCGCAGCACATGCACTGGA
AACGGATTCTGTCCTCGTTACCAATGACGCCGCATTTAGTCGTGTTGTTGGTTTGACCGTGGAAGATTGGACACGCAGCT
AA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT141507 WP_001307405.1 NZ_CP045213:4399649-4399984 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT141507 NZ_CP059993:55849-56109 [Xanthomonas citri]
GTGAGCCAAGTTGCCAAGCTGTTCATCAATGGCCGCAGCCAGGCAGTCCGCTTGCCTGCCGCCTATCGCTTTACCGAGAA
AGAGGTTTTCATTCGCCAAGACCCGGAGACCGGTGACGTCATCCTGTCTCGGAAGCCTGCAACGTGGGAGGACTTCTTTA
TCGTACTCAAGAGCGCAGATGTGCCTGCTAATTTTCTAGATGCCGCCGAGCGCAATCAGGGCACGCAGGACCGTGATCCT
TTTGCGGGATGGCGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References