Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1094422..1094963 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GC231_RS05230 Protein ID WP_000615976.1
Coordinates 1094422..1094700 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GC231_RS05235 Protein ID WP_000729704.1
Coordinates 1094703..1094963 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS05215 (1092004) 1092004..1092582 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GC231_RS05220 (1092788) 1092788..1093555 + 768 WP_000333380.1 class II glutamine amidotransferase -
GC231_RS05225 (1093526) 1093526..1094266 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
GC231_RS05230 (1094422) 1094422..1094700 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GC231_RS05235 (1094703) 1094703..1094963 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GC231_RS05240 (1095149) 1095149..1095922 + 774 WP_000543895.1 C40 family peptidase -
GC231_RS05245 (1095979) 1095979..1096335 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
GC231_RS05250 (1096328) 1096328..1096606 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GC231_RS05255 (1096711) 1096711..1098423 - 1713 Protein_1007 flagellar biosynthesis protein FlhA -
GC231_RS05260 (1098395) 1098395..1099180 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1085473..1096606 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T141488 WP_000615976.1 NZ_CP045213:c1094700-1094422 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T141488 NZ_CP059963:c3895104-3894688 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAGTGCACGCTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCTGGCACACCTATTGGTCCAAACGATACGGCCATCGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT141488 WP_000729704.1 NZ_CP045213:c1094963-1094703 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT141488 NZ_CP059963:c3895328-3895101 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAGCCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCCGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References