Detailed information of TA system TA141485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 155449..156051
• Replicon: chromosome
• Accession: NZ_CP045213
• Organism: Escherichia coli strain EH41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GC231_RS00755
• Protein ID: WP_000897305.1
• Coordinates: 155449..155760 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GC231_RS00760
• Protein ID: WP_000356397.1
• Coordinates: 155761..156051 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC231_RS00730 (151363)	151363..151962	+	600	WP_001295269.1	glucose-1-phosphatase	-
GC231_RS00735 (151956)	151956..152828	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GC231_RS00740 (152825)	152825..153262	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
GC231_RS00745 (153307)	153307..154248	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GC231_RS00750 (154312)	154312..155220	-	909	WP_001162704.1	alpha/beta hydrolase	-
GC231_RS00755 (155449)	155449..155760	+	312	WP_000897305.1	hypothetical protein	Toxin
GC231_RS00760 (155761)	155761..156051	+	291	WP_000356397.1	NadS family protein	Antitoxin
GC231_RS00765 (156656)	156656..156874	+	219	WP_001295676.1	CopG family transcriptional regulator	-
GC231_RS00770 (157093)	157093..157335	+	243	WP_001087409.1	protein YiiF	-
GC231_RS00775 (157665)	157665..158594	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
GC231_RS00780 (158591)	158591..159226	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GC231_RS00785 (159223)	159223..160125	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T141485 WP_000897305.1 NZ_CP045213:155449-155760 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T141485 NZ_CP059963:c1214882-1214679 [Pectobacterium brasiliense]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGCCTTGCAGAACTGACGATGAACAAAC
TCTACGACAAAGTCCCTACTGCAGTATGGCGATACGTTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT141485 WP_000356397.1 NZ_CP045213:155761-156051 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT141485 NZ_CP059963:c1215304-1214936 [Pectobacterium brasiliense]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
GACGTTAGGCGATAGCAGTCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGTATTAATGATGCTGAACTACGGCGTTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACATCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7