Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4267138..4267937 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag GC226_RS20710 Protein ID WP_000347266.1
Coordinates 4267473..4267937 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GC226_RS20705 Protein ID WP_001307405.1
Coordinates 4267138..4267473 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS20690 (4262923) 4262923..4263693 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GC226_RS20695 (4263709) 4263709..4265043 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GC226_RS20700 (4265418) 4265418..4266989 + 1572 WP_001273741.1 galactarate dehydratase -
GC226_RS20705 (4267138) 4267138..4267473 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GC226_RS20710 (4267473) 4267473..4267937 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GC226_RS20715 (4267992) 4267992..4268801 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GC226_RS20720 (4269050) 4269050..4270330 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
GC226_RS20725 (4270353) 4270353..4270826 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GC226_RS20730 (4270837) 4270837..4271616 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
GC226_RS20735 (4271606) 4271606..4272484 + 879 WP_228400141.1 PTS N-acetylgalactosamine transporter subunit IID -
GC226_RS20740 (4272502) 4272502..4272936 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4256376..4267937 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T141477 WP_000347266.1 NZ_CP045209:4267473-4267937 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T141477 NZ_CP059962:c5034389-5034084 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT141477 WP_001307405.1 NZ_CP045209:4267138-4267473 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT141477 NZ_CP059962:c5034610-5034392 [Pectobacterium brasiliense]
ATGAAACACCGCGTTAGTGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCAGATGAAAACAGGAATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References