Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1015049..1015590 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GC226_RS04760 Protein ID WP_000615976.1
Coordinates 1015049..1015327 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GC226_RS04765 Protein ID WP_000729704.1
Coordinates 1015330..1015590 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS04745 (1012631) 1012631..1013209 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GC226_RS04750 (1013415) 1013415..1014182 + 768 WP_000333380.1 class II glutamine amidotransferase -
GC226_RS04755 (1014153) 1014153..1014893 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
GC226_RS04760 (1015049) 1015049..1015327 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GC226_RS04765 (1015330) 1015330..1015590 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GC226_RS04770 (1015776) 1015776..1016549 + 774 WP_000543895.1 C40 family peptidase -
GC226_RS04775 (1016606) 1016606..1016962 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
GC226_RS04780 (1016955) 1016955..1017233 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GC226_RS04785 (1017338) 1017338..1019050 - 1713 Protein_915 flagellar biosynthesis protein FlhA -
GC226_RS04790 (1019022) 1019022..1019807 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1006100..1017233 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T141459 WP_000615976.1 NZ_CP045209:c1015327-1015049 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T141459 NZ_CP059961:3072390-3072704 [Pectobacterium brasiliense]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATCATTGG
GCAATTGAATCGTCGGATAATGATCCCATTGCTCCCAATTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCCTGGGT
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAAGCTTCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT141459 WP_000729704.1 NZ_CP045209:c1015590-1015330 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT141459 NZ_CP059961:3072151-3072387 [Pectobacterium brasiliense]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATCAATCTGAGTGCTACCCTGACAACCGCTCTGGATGCAGAGCTTCGCTCTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTCAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References