Detailed information of TA system TA141456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 155431..156033
• Replicon: chromosome
• Accession: NZ_CP045209
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GC226_RS00755
• Protein ID: WP_000897305.1
• Coordinates: 155431..155742 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GC226_RS00760
• Protein ID: WP_000356397.1
• Coordinates: 155743..156033 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS00730 (151345)	151345..151944	+	600	WP_001295269.1	glucose-1-phosphatase	-
GC226_RS00735 (151938)	151938..152810	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GC226_RS00740 (152807)	152807..153244	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
GC226_RS00745 (153289)	153289..154230	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GC226_RS00750 (154294)	154294..155202	-	909	WP_001162704.1	alpha/beta hydrolase	-
GC226_RS00755 (155431)	155431..155742	+	312	WP_000897305.1	hypothetical protein	Toxin
GC226_RS00760 (155743)	155743..156033	+	291	WP_000356397.1	NadS family protein	Antitoxin
GC226_RS00765 (156638)	156638..156856	+	219	WP_001295676.1	CopG family transcriptional regulator	-
GC226_RS00770 (157075)	157075..157317	+	243	WP_001087409.1	protein YiiF	-
GC226_RS00775 (157647)	157647..158576	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
GC226_RS00780 (158573)	158573..159208	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GC226_RS00785 (159205)	159205..160107	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T141456 WP_000897305.1 NZ_CP045209:155431-155742 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T141456 NZ_CP059961:1785937-1786350 [Pectobacterium brasiliense]
GTGGCCCAGTGGCGATGTGACCTCCGCGTTTCCTGGCGCATGCAGCTGTTATCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAATCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCATATTACTGTCATTGCAAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGCTGGCATCGGACAGCATGGGTGATGACGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT141456 WP_000356397.1 NZ_CP045209:155743-156033 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT141456 NZ_CP059961:1785690-1785956 [Pectobacterium brasiliense]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCAATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7