Detailed information of TA system TA141449

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 144397..144822
• Replicon: plasmid unnamed1
• Accession: NZ_CP045207
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: GBG22_RS24775
• Protein ID: WP_001332444.1
• Coordinates: 144397..144558 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	144611..144822 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS24745	139747..139974	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
GBG22_RS24750	140068..140754	-	687	WP_000332490.1	PAS domain-containing protein	-
GBG22_RS24755	140944..141327	-	384	WP_000124981.1	relaxosome protein TraM	-
GBG22_RS24760	141661..142251	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
GBG22_RS24765	142547..143368	-	822	WP_001234485.1	DUF945 domain-containing protein	-
GBG22_RS24770	143547..144159	+	613	Protein_153	hypothetical protein	-
GBG22_RS24775	144397..144558	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	144611..144822	-	212	NuclAT_0	-	Antitoxin
-	144611..144822	-	212	NuclAT_0	-	Antitoxin
-	144611..144822	-	212	NuclAT_0	-	Antitoxin
-	144611..144822	-	212	NuclAT_0	-	Antitoxin
GBG22_RS24780	144833..145552	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
GBG22_RS24785	145549..145983	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
GBG22_RS24790	146038..147996	-	1959	Protein_157	ParB/RepB/Spo0J family partition protein	-
GBG22_RS24795	148055..148288	-	234	WP_001209608.1	DUF905 domain-containing protein	-
GBG22_RS24800	148351..148848	-	498	WP_050575774.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..194162	194162
	-	flank	IS/Tn	-	-	149694..150197	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T141449 WP_001332444.1 NZ_CP045207:c144558-144397 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T141449 NZ_CP059961:c924687-923368 [Pectobacterium brasiliense]
ATGGCTGCTCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATACGAACTGGCTCGGATTGCTGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAT
ACGGATTCCACCCAACCGTTTGATCTGCTCGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATATAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATT
TGTCCGATGCCCCGCTGGGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCA
TTGCTTTATCTGGATGATCGCTGGTGCCTGCCTCTCAATGTCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTAGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTTATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGACTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCCAAGGTCGTTGGGTTCGCCAGAGAATCGATGGAAAACATTCTGGCGGAGTTTGCCCAGACCGTT
GATACCGTCATGACGACTGTTGGAAGTCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin

Nucleotide

• Length: 212 bp

>AT141449 NZ_CP045207:c144822-144611 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin