Detailed information of TA system TA141445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4730854..4731456
• Replicon: chromosome
• Accession: NZ_CP045206
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: GBG22_RS22990
• Protein ID: WP_000897302.1
• Coordinates: 4731145..4731456 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBG22_RS22985
• Protein ID: WP_000356397.1
• Coordinates: 4730854..4731144 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS22960	4726927..4727829	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GBG22_RS22965	4727826..4728461	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GBG22_RS22970	4728458..4729387	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
GBG22_RS22975	4729603..4729821	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
GBG22_RS22980	4730217..4730495	-	279	WP_001296612.1	hypothetical protein	-
GBG22_RS22985	4730854..4731144	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GBG22_RS22990	4731145..4731456	-	312	WP_000897302.1	hypothetical protein	Toxin
GBG22_RS22995	4731685..4732593	+	909	WP_001331553.1	alpha/beta hydrolase	-
GBG22_RS23000	4732657..4733598	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GBG22_RS23005	4733643..4734080	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GBG22_RS23010	4734077..4734949	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GBG22_RS23015	4734943..4735542	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T141445 WP_000897302.1 NZ_CP045206:c4731456-4731145 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T141445 NZ_CP059961:734541-734720 [Pectobacterium brasiliense]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACCGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT141445 WP_000356397.1 NZ_CP045206:c4731144-4730854 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT141445 NZ_CP059961:734748-735158 [Pectobacterium brasiliense]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCCACCGTGTCTTCAGTAGGCCAATTGGCAAAAAGCGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCACTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAAGCGGCATTAGAGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: S1P416