Detailed information of TA system TA141439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3748527..3749221
• Replicon: chromosome
• Accession: NZ_CP045206
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: GBG22_RS18425
• Protein ID: WP_001263500.1
• Coordinates: 3748527..3748925 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GBG22_RS18430
• Protein ID: WP_000554758.1
• Coordinates: 3748928..3749221 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS18400	3743892..3744350	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
GBG22_RS18405	3744611..3746068	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
GBG22_RS18410	3746125..3746739	-	615	WP_000602123.1	peptide chain release factor H	-
GBG22_RS18415	3746736..3747875	-	1140	WP_039267783.1	RNA ligase RtcB family protein	-
GBG22_RS18420	3748065..3748517	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
GBG22_RS18425	3748527..3748925	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GBG22_RS18430	3748928..3749221	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GBG22_RS18435	3749273..3750328	-	1056	WP_001226168.1	DNA polymerase IV	-
GBG22_RS18440	3750399..3751184	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
GBG22_RS18445	3751156..3752868	+	1713	Protein_3612	flagellar biosynthesis protein FlhA	-
GBG22_RS18450	3752947..3753105	+	159	WP_014639450.1	hypothetical protein	-
GBG22_RS18455	3753188..3753685	-	498	WP_039267782.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3748527..3768881	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T141439 WP_001263500.1 NZ_CP045206:c3748925-3748527 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T141439 NZ_CP059960:2091543-2091956 [Pectobacterium brasiliense]
GTGGCCCAGTGGCGATGTGACCTCCGCGTTTCCTGGCGCATGCAGCTGTTATCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAATCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCATATTACTGTCATTGCAAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGCTGGCATCGGACAGCATGGGTGATGACGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT141439 WP_000554758.1 NZ_CP045206:c3749221-3748928 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT141439 NZ_CP059960:2091296-2091562 [Pectobacterium brasiliense]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCAATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3