Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3502023..3502702 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag GBG22_RS17225 Protein ID WP_000057524.1
Coordinates 3502400..3502702 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag GBG22_RS17220 Protein ID WP_000806442.1
Coordinates 3502023..3502364 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS17210 3498267..3499199 - 933 WP_000883052.1 glutaminase A -
GBG22_RS17215 3499461..3501965 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
GBG22_RS17220 3502023..3502364 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
GBG22_RS17225 3502400..3502702 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GBG22_RS17230 3502835..3503629 + 795 WP_000365177.1 TraB/GumN family protein -
GBG22_RS17235 3503833..3504312 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
GBG22_RS17240 3504336..3505136 + 801 WP_000439798.1 hypothetical protein -
GBG22_RS17245 3505133..3505636 + 504 WP_000667000.1 hypothetical protein -
GBG22_RS17250 3505674..3507326 - 1653 WP_021512190.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3494712..3505636 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T141437 WP_000057524.1 NZ_CP045206:c3502702-3502400 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T141437 NZ_CP059960:1934853-1935347 [Pectobacterium brasiliense]
ATGATCTCCGCTCCTGAGCCACTCCGCGCCGAACATATACTATCCTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACGAATGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAAGGTGTTGGACGGGCATTGGTAAGAGATGC
TGGGCTACGGGTGATTCAGGTTGCGGATACTATCGGTATTCGCGGGATGTTAGTTCATGCGCTGTCTGACGAAGCGCGGG
AGTTTTACCTACGAGTGGGGTTTGAGCCTTCGCCGATTGACCCGATGATGTTGATGGTGACGTTAGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT141437 WP_000806442.1 NZ_CP045206:c3502364-3502023 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT141437 NZ_CP059960:1934563-1934856 [Pectobacterium brasiliense]
ATGCCAGCGACGAATAGCGTTTCTGTTAAACGTGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGACCTTATCGA
CCGTGCGGCGAAGGCCAGAGGAAAAAATCGCACTGATTTTGTCCTCGAAGCTGCTCGTGCCGCTGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGCCTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGAAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References