Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2320936..2321498 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag GBG22_RS11210 Protein ID WP_000605675.1
Coordinates 2321220..2321498 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag GBG22_RS11205 Protein ID WP_000781370.1
Coordinates 2320936..2321220 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS11190 2316117..2319164 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
GBG22_RS11195 2319177..2320061 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
GBG22_RS11200 2320054..2320707 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
GBG22_RS11205 2320936..2321220 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
GBG22_RS11210 2321220..2321498 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GBG22_RS11215 2321684..2322694 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
GBG22_RS11220 2322828..2324525 - 1698 WP_000433462.1 malate dehydrogenase -
GBG22_RS11225 2324681..2324818 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
GBG22_RS11230 2324920..2325135 - 216 WP_000495766.1 biofilm-dependent modulation protein -
GBG22_RS11235 2325480..2325911 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T141427 WP_000605675.1 NZ_CP045206:c2321498-2321220 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T141427 NZ_CP059960:c422663-422247 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAGTGCACGCTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCTGGCACACCTATTGGTCCAAACGATACGGCCATCGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT141427 WP_000781370.1 NZ_CP045206:c2321220-2320936 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT141427 NZ_CP059960:c422887-422660 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAGCCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCCGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References