Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1895337..1895839 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GBG22_RS09115 Protein ID WP_000767829.1
Coordinates 1895585..1895839 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GBG22_RS09110 Protein ID WP_001259255.1
Coordinates 1895337..1895588 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS09085 1890515..1891582 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GBG22_RS09090 1891582..1892652 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
GBG22_RS09095 1892649..1893953 - 1305 WP_039267630.1 histidinol dehydrogenase -
GBG22_RS09100 1893959..1894858 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
GBG22_RS09105 1895004..1895054 - 51 WP_071593552.1 his operon leader peptide -
GBG22_RS09110 1895337..1895588 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GBG22_RS09115 1895585..1895839 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GBG22_RS09120 1895922..1896746 + 825 WP_000754737.1 SDR family oxidoreductase -
GBG22_RS09125 1896792..1897721 + 930 WP_000803351.1 LysR family transcriptional regulator -
GBG22_RS09130 1897936..1897998 + 63 WP_010723108.1 membrane protein YoeI -
GBG22_RS09135 1897988..1899346 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GBG22_RS09140 1899525..1900583 + 1059 WP_023568741.1 transport protein YeeE -
GBG22_RS09145 1900597..1900824 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T141421 WP_000767829.1 NZ_CP045206:1895585-1895839 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T141421 NZ_CP059959:1528281-1528763 [Pectobacterium brasiliense]
ATGGGAATAACTGCGCCAGAGCCATTATCTTCCAGCCATAATCTTGCTGAATTTTGCAGCCAAGAACCGAGCTTGAATGA
ATGGCTAAAAAAGAAAGCGCTAAAAAATCATAGTGCAGGGGTTTCTCGTGTATATGTGATTTGCGCTGAAGGCACACATC
AGGTGATTGGATATTATTGTTTATCAACAGGTAGCGTTCAGAGAAATACAGCGCCTGGTGCATATAGACGTAATGCCCCC
GAATCACTACCAGTCATTGTCCTTGGGCGGCTTGCCATTGATCAGGCGTATTCTGGTAAAGGTTTAGGTGTGGCTTTATT
AAAAGACGCGATATTTCGGACTGAGAATATTGCTTTGCAAGTGGGTGTGAGGGCATTAGTCGTTCACGCGCTGGATGAGA
ACGTGCGCAATTTCTACCTTAAATTCGCTTTTGATCCCTCGCCAGTGCAGCCCCTGATGCTACTCTATCCAATTAAAATT
TAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT141421 WP_001259255.1 NZ_CP045206:1895337-1895588 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT141421 NZ_CP059959:1528012-1528290 [Pectobacterium brasiliense]
ATGAAACCTGAAACTAAAGAAGCGCCAATCAATATTCGGGCCAAAGCCTCCCAACGGGATTTGATCGATATGGCTGCAAA
CCTTGTGTCAAAATCGCGTACCGATTTTATGCTTGAAGCAGCCTGCCGCGAGGCGCAGGACATTTTGTTGGATCAACGTT
TGTTTATTTTGGATAACGAACAGTTTGACGAATTTTTGGCCGAGTTGGATGCGCCTATCACCGCAGAGCGTCAGGCTCGC
ATTAACGATCTAATGAGCCGCAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References