Detailed information of TA system TA141389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3711805..3712499
• Replicon: chromosome
• Accession: NZ_CP045190
• Organism: Escherichia coli strain NT1F31

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GAG93_RS19050
• Protein ID: WP_001263493.1
• Coordinates: 3711805..3712203 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GAG93_RS19055
• Protein ID: WP_000554757.1
• Coordinates: 3712206..3712499 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAG93_RS19020	3706805..3708049	-	1245	WP_000189541.1	esterase FrsA	-
-	3707465..3707545	-	81	NuclAT_9	-	-
-	3707465..3707545	-	81	NuclAT_9	-	-
-	3707465..3707545	-	81	NuclAT_9	-	-
-	3707465..3707545	-	81	NuclAT_9	-	-
GAG93_RS19025	3708141..3708599	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GAG93_RS19030	3708860..3710317	+	1458	WP_122057011.1	cytosol nonspecific dipeptidase	-
GAG93_RS19035	3710374..3710895	-	522	Protein_3519	peptide chain release factor H	-
GAG93_RS19040	3710894..3711097	-	204	Protein_3520	RNA ligase RtcB family protein	-
GAG93_RS19045	3711343..3711795	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GAG93_RS19050	3711805..3712203	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GAG93_RS19055	3712206..3712499	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GAG93_RS19060	3712551..3713606	-	1056	WP_001226164.1	DNA polymerase IV	-
GAG93_RS19065	3713677..3714462	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
GAG93_RS19070	3714434..3716146	+	1713	Protein_3526	flagellar biosynthesis protein FlhA	-
GAG93_RS19075	3716370..3716867	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T141389 WP_001263493.1 NZ_CP045190:c3712203-3711805 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T141389 NZ_CP059957:c255784-255497 [Pectobacterium brasiliense]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGCTAGGGCATCCTGTCCGGGAACAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGCGAAGGTAGCCAGATTTATTCTGATACGAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT141389 WP_000554757.1 NZ_CP045190:c3712499-3712206 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT141389 NZ_CP059957:c256028-255774 [Pectobacterium brasiliense]
ATGGCTTTCAACATCCTAACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTTCGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCTGTATTCGCCTATTTAA
TGGAACTGGCTGAAGATGCAGAACTGGGGCGTACCGTTGATGATCGGATGTCAGAGCTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6