Detailed information of TA system TA141367

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95690..95954
• Replicon: plasmid pNT1F31-96kb
• Accession: NZ_CP045189
• Organism: Escherichia coli strain NT1F31

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: GAG93_RS01130
• Protein ID: WP_001331364.1
• Coordinates: 95690..95842 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	95897..95954 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAG93_RS01105	91156..93324	+	2169	WP_156262330.1	IncI1-type conjugal transfer membrane protein TraY	-
GAG93_RS01110	93400..94014	+	615	WP_156262331.1	plasmid IncI1-type surface exclusion protein ExcA	-
GAG93_RS01115	94112..94321	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
GAG93_RS24375	94530..94706	+	177	WP_001054904.1	hypothetical protein	-
GAG93_RS01120	94771..95067	-	297	WP_000115013.1	hypothetical protein	-
GAG93_RS01125	95367..95618	+	252	WP_001535703.1	hypothetical protein	-
GAG93_RS01130	95690..95842	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	95897..95954	+	58	NuclAT_0	-	Antitoxin
-	95897..95954	+	58	NuclAT_0	-	Antitoxin
-	95897..95954	+	58	NuclAT_0	-	Antitoxin
-	95897..95954	+	58	NuclAT_0	-	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..96046	96046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T141367 WP_001331364.1 NZ_CP045189:c95842-95690 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T141367 NZ_CP059955:1866942-1867247 [Pectobacterium brasiliense]
ATGCCACAAGTGATTGTTTCAGAACGTGCAAGACGTGATTTGCAACGTCTGCAGGATTTTCTAAAAAACAAAAATGCGCT
GGCTGCGAAAAAGGCGGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCCGTTG
AACATCTCCCTCTTGAATATCAGGAACTGGTGATCGAATTCGGCAGCAGCGGGTACGTGGTGCTTTACCGCCATGATGAA
CTGACTGACAGAGTGGTGATCCTGACCGTCAAGCATCAAAAAGAAGCCGGTTACCAAAACCCTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT141367 NZ_CP045189:95897-95954 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin