Detailed information of TA system TA141329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 679..1319
• Replicon: plasmid pPCP
• Accession: NZ_CP045157
• Organism: Yersinia pestis strain SCPM-O-B-5935 (I-1996)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GCK71_RS22645
• Protein ID: WP_002225538.1
• Coordinates: 978..1319 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: GCK71_RS22640
• Protein ID: WP_002213446.1
• Coordinates: 679..978 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCK71_RS22640	679..978	-	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
GCK71_RS22645	978..1319	-	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCK71_RS22655	2586..3608	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
GCK71_RS22660	3605..4387	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
GCK71_RS22665	5424..5618	+	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla	1..9611	9611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T141329 WP_002225538.1 NZ_CP045157:c1319-978 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T141329 NZ_CP059947:300402-300929 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT141329 WP_002213446.1 NZ_CP045157:c978-679 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT141329 NZ_CP059947:300129-300395 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016