Detailed information of TA system TA141314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 24097..24605
• Replicon: plasmid pTP33
• Accession: NZ_CP045152
• Organism: Yersinia pestis strain SCPM-O-DNA-18 (I-3113)

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A0K1H0I1
• Locus tag: GCK70_RS22535
• Protein ID: WP_016257350.1
• Coordinates: 24345..24605 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A1E7YXI3
• Locus tag: GCK70_RS22530
• Protein ID: WP_016257351.1
• Coordinates: 24097..24348 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCK70_RS22505	20649..20987	-	339	WP_016257047.1	hypothetical protein	-
GCK70_RS22510	20984..21202	-	219	WP_016257048.1	ANR family transcriptional regulator	-
GCK70_RS22515	21424..22371	-	948	WP_016257049.1	replication initiation protein	-
GCK70_RS22520	22818..23645	+	828	WP_134809573.1	DUF4236 domain-containing protein	-
GCK70_RS22525	23674..24039	+	366	WP_016257352.1	hypothetical protein	-
GCK70_RS22530	24097..24348	+	252	WP_016257351.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
GCK70_RS22535	24345..24605	+	261	WP_016257350.1	Txe/YoeB family addiction module toxin	Toxin
GCK70_RS22540	24608..25168	+	561	WP_016257349.1	3'-5' exoribonuclease	-
GCK70_RS22545	25147..25362	-	216	WP_071588212.1	WGR domain-containing protein	-
GCK70_RS22550	25495..25722	-	228	WP_016257348.1	hypothetical protein	-
GCK70_RS22555	25719..26342	-	624	WP_016257347.1	ParA family protein	-
GCK70_RS22560	26962..27264	+	303	WP_016257346.1	phage holin family protein	-
GCK70_RS22565	27266..27691	+	426	WP_025476582.1	lysozyme	-
GCK70_RS22570	27688..28209	+	522	WP_016257344.1	DUF2514 domain-containing protein	-
GCK70_RS22575	28263..29264	+	1002	WP_016257343.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33990	33990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10232.70 Da
• Isoelectric Point: 9.5040

>T141314 WP_016257350.1 NZ_CP045152:24345-24605 [Yersinia pestis]
VKLVFSDQSWADYLHWQQTDKSMLKRVNLLIKDIQREPFSGMGKPEPLKHHLSGFWSRRIDSEHRLVYRVTDDSLLIASC
RYHYTG

Nucleotide

• Length: 261 bp

>T141314 NZ_CP059929:c1472768-1472370 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9495.74 Da
• Isoelectric Point: 4.4488

>AT141314 WP_016257351.1 NZ_CP045152:24097-24348 [Yersinia pestis]
MKTMNYSEARQNFASVMNETIDDRAPILITRQKGMPCVLMSLEEYDSLEETAYLLRSPVNARHLMDSIEQLNNGKGTERD
LAE

Nucleotide

• Length: 252 bp

>AT141314 NZ_CP059929:c1473064-1472771 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0K1H0I1

Antitoxin

• AlphaFold DB: A0A1E7YXI3