Detailed information of TA system TA141296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3610956..3611650
• Replicon: chromosome
• Accession: NZ_CP045145
• Organism: Yersinia pestis strain SCPM-O-B-6899 (231)

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: A0A0E1NW62
• Locus tag: GCK69_RS16925
• Protein ID: WP_002214787.1
• Coordinates: 3611354..3611650 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q666X1
• Locus tag: GCK69_RS16920
• Protein ID: WP_002209924.1
• Coordinates: 3610956..3611354 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCK69_RS16890	3606144..3606509	+	366	WP_002209919.1	hypothetical protein	-
GCK69_RS16895	3606522..3606758	+	237	WP_002214792.1	hypothetical protein	-
GCK69_RS16900	3606764..3607096	+	333	WP_002214791.1	hypothetical protein	-
GCK69_RS16905	3607278..3607478	+	201	WP_002209920.1	AlpA family transcriptional regulator	-
GCK69_RS22965	3607492..3607644	-	153	WP_002209921.1	hypothetical protein	-
GCK69_RS16910	3607637..3607963	-	327	WP_002209922.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GCK69_RS16915	3608263..3610554	+	2292	WP_002209923.1	toprim domain-containing protein	-
GCK69_RS16920	3610956..3611354	-	399	WP_002209924.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
GCK69_RS16925	3611354..3611650	-	297	WP_002214787.1	type II toxin-antitoxin system MqsR family toxin	Toxin
GCK69_RS16930	3611814..3612176	-	363	WP_002209925.1	helix-turn-helix domain-containing protein	-
GCK69_RS16935	3612239..3612499	-	261	WP_002214785.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GCK69_RS16940	3613097..3613282	+	186	WP_002209928.1	hypothetical protein	-
GCK69_RS16945	3613364..3614578	-	1215	WP_002209929.1	tyrosine-type recombinase/integrase	-
GCK69_RS16955	3615011..3616528	-	1518	WP_002209930.1	lysine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3593120..3619548	26428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11319.12 Da
• Isoelectric Point: 7.8810

>T141296 WP_002214787.1 NZ_CP045145:c3611650-3611354 [Yersinia pestis]
MEKRTPHTRLLKVKELVLKGNIKTTRTARDGAEELGLSFRDMCDAVSELISADFYKSMTTHQDHTIWQDVYRPMLSCGRV
YLKITVIDDVLNVSFKEI

Nucleotide

• Length: 297 bp

>T141296 NZ_CP059917:c4511528-4511151 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14657.17 Da
• Isoelectric Point: 8.4923

>AT141296 WP_002209924.1 NZ_CP045145:c3611354-3610956 [Yersinia pestis]
MMKCPVCGGVELIHEARDVPFTYKGRKFIVEGVIGQHCPACGETVMTEAESDVYAAKTMLLRKMVNTESIAPAYIVQIRK
KLGLNQREASEIFGGGVNAFSRYEKGKALPHPSTIKLLQVLDKHPELLNEIR

Nucleotide

• Length: 399 bp

>AT141296 NZ_CP059917:c4511746-4511525 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E1NW62

Antitoxin

• AlphaFold DB: Q666X1