Detailed information of TA system TA141266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 846364..847015
• Replicon: chromosome
• Accession: NZ_CP045135
• Organism: Acinetobacter indicus strain XG01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: GA636_RS04040
• Protein ID: WP_004668307.1
• Coordinates: 846659..847015 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GA636_RS04035
• Protein ID: WP_001140620.1
• Coordinates: 846364..846666 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GA636_RS04010	842135..842839	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
GA636_RS04015	842903..844183	-	1281	WP_005237495.1	cation transporter	-
GA636_RS04020	844273..844665	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
GA636_RS04025	844851..846155	+	1305	WP_160232240.1	IS4 family transposase	-
GA636_RS04030	846137..846322	+	186	Protein_765	IS6 family transposase	-
GA636_RS04035	846364..846666	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
GA636_RS04040	846659..847015	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GA636_RS04045	847538..848509	-	972	WP_005176780.1	helix-turn-helix domain-containing protein	-
GA636_RS04050	848642..849526	+	885	WP_160232241.1	MBL fold metallo-hydrolase	-
GA636_RS04055	849611..850153	+	543	WP_004637127.1	cysteine hydrolase	-
GA636_RS04060	850228..850521	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GA636_RS04065	850511..850756	-	246	WP_160232242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	842135..855702	13567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T141266 WP_004668307.1 NZ_CP045135:c847015-846659 [Acinetobacter indicus]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T141266 NZ_CP059907:4484883-4485101 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT141266 WP_001140620.1 NZ_CP045135:c846666-846364 [Acinetobacter indicus]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT141266 NZ_CP059907:4484483-4484857 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: N9NM16