Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31476..32169 Replicon plasmid p18TQ-X3
Accession NZ_CP045132
Organism Acinetobacter indicus strain TQ18

Toxin (Protein)


Gene name higB Uniprot ID R9B971
Locus tag GA721_RS14230 Protein ID WP_016165531.1
Coordinates 31879..32169 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6N0LK42
Locus tag GA721_RS14225 Protein ID WP_035268402.1
Coordinates 31476..31877 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GA721_RS14210 27165..27944 - 780 WP_150378261.1 ParA family protein -
GA721_RS14215 28801..29973 + 1173 WP_150378260.1 replication initiation protein -
GA721_RS14220 30129..31348 + 1220 WP_104425813.1 IS3 family transposase -
GA721_RS14225 31476..31877 - 402 WP_035268402.1 helix-turn-helix domain-containing protein Antitoxin
GA721_RS14230 31879..32169 - 291 WP_016165531.1 type II toxin-antitoxin system HigB family toxin Toxin
GA721_RS14235 32335..32589 + 255 Protein_38 transposase -
GA721_RS14240 32640..33185 - 546 WP_127800924.1 DNA-binding protein -
GA721_RS14245 33291..33776 + 486 Protein_40 IS3 family transposase -
GA721_RS14250 33826..34338 - 513 WP_150378258.1 signal peptidase II -
GA721_RS14255 34342..35238 - 897 WP_150378257.1 cation transporter -
GA721_RS14260 35334..35741 + 408 WP_001219642.1 Cd(II)/Pb(II)-responsive transcriptional regulator -
GA721_RS14265 35987..36640 - 654 Protein_44 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(X3) / aph(3'')-Ib / msr(E) / mph(E) / aph(3')-Ia / aac(3)-IId / blaOXA-58 / sul2 - 1..93847 93847
- inside IScluster/Tn tet(X3) / aph(3'')-Ib / msr(E) / mph(E) / aph(3')-Ia - 2988..62112 59124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(19-90)

Antitoxin

Antitoxin HigA

(11-133)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11257.12 Da        Isoelectric Point: 10.1259

>T141265 WP_016165531.1 NZ_CP045132:c32169-31879 [Acinetobacter indicus]
MHVITHARIIEAMHKWPQAETALDGWYRTIKANDPKDFAEMKQLFPAVDKVGKFHVFDIGGNKIRLIAVVMYQAKRVYIR
HVLSHKEYDKGHWKEG

Download         Length: 291 bp

>T141265 NZ_CP059907:c4457131-4456829 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14823.65 Da        Isoelectric Point: 4.3676

>AT141265 WP_035268402.1 NZ_CP045132:c31877-31476 [Acinetobacter indicus]
MNAMIKQAVDHWHYVAPLLSKPENEDDFHTLVEALDELLDTVGDDESNPLMGLIHQLGDLVSDYENEHLPMPKGDGRAAL
AFLMEQHGLGQSDLAEVATQSVISEILSGKRQLNIRHIKALSERFNVSADTFF

Download         Length: 402 bp

>AT141265 NZ_CP059907:c4456793-4456452 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9B971

Antitoxin

Source ID Structure
AlphaFold DB A0A6N0LK42

References