Detailed information of TA system TA141252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3630517..3631099
• Replicon: chromosome
• Accession: NZ_CP045119
• Organism: Rubrobacter sp. SCSIO 52909

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GBA63_RS18245
• Protein ID: WP_207956898.1
• Coordinates: 3630860..3631099 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBA63_RS18240
• Protein ID: WP_207956897.1
• Coordinates: 3630517..3630849 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBA63_RS18225	3625982..3626818	-	837	WP_166178392.1	shikimate dehydrogenase	-
GBA63_RS18230	3626865..3630053	-	3189	WP_207956896.1	DEAD/DEAH box helicase family protein	-
GBA63_RS18235	3630091..3630492	-	402	WP_166178394.1	hypothetical protein	-
GBA63_RS18240	3630517..3630849	-	333	WP_207956897.1	HigA family addiction module antidote protein	Antitoxin
GBA63_RS18245	3630860..3631099	-	240	WP_207956898.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBA63_RS18250	3631194..3634136	-	2943	WP_166178398.1	site-specific DNA-methyltransferase	-
GBA63_RS18255	3634256..3635008	-	753	WP_166178400.1	hypothetical protein	-
GBA63_RS18260	3635208..3635705	-	498	WP_166178402.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-79)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (15-106)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9131.25 Da
• Isoelectric Point: 9.0875

>T141252 WP_207956898.1 NZ_CP045119:c3631099-3630860 [Rubrobacter sp. SCSIO 52909]
MGRKSRAIPEQARRRAQVKLAQLNRVESVEELARPPGNRLHKLSGDREGQWSVSINARYRICFLFEDGDAHDVEVTDYH

Nucleotide

• Length: 240 bp

>T141252 NZ_CP059907:c1563836-1563462 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12629.28 Da
• Isoelectric Point: 4.4331

>AT141252 WP_207956897.1 NZ_CP045119:c3630849-3630517 [Rubrobacter sp. SCSIO 52909]
MGEWEKEISAEERVYPPVHPGEVLRQEWLEPLEMNPNRLAKALGVDRQNVYEIVHGRRAVSADMALRLGRWSGMRPGFWL
GLQADYDLQMAEWERGDEIAEQVEPLVTAG

Nucleotide

• Length: 333 bp

>AT141252 NZ_CP059907:c1564294-1563926 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA