Detailed information of TA system TA141236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2613..3177
• Replicon: plasmid pAJ4134
• Accession: NZ_CP045107
• Organism: Acinetobacter johnsonii strain AJ413

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GBN93_RS18120
• Protein ID: WP_234966663.1
• Coordinates: 2878..3177 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBN93_RS18115
• Protein ID: WP_234966661.1
• Coordinates: 2613..2885 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN93_RS18115 (GBN93_18145)	2613..2885	-	273	WP_234966661.1	NadS family protein	Antitoxin
GBN93_RS18120 (GBN93_18150)	2878..3177	-	300	WP_234966663.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBN93_RS18125 (GBN93_18155)	3306..3585	+	280	Protein_3	IS982 family transposase	-
GBN93_RS18130 (GBN93_18165)	4495..4809	+	315	WP_234966649.1	hypothetical protein	-
GBN93_RS18135	4837..5007	-	171	WP_234966651.1	hypothetical protein	-
GBN93_RS18140 (GBN93_18170)	5004..5390	-	387	WP_234966653.1	hypothetical protein	-
GBN93_RS18145 (GBN93_18180)	6597..7514	+	918	WP_234966655.1	replication initiation protein RepM	-
GBN93_RS18150 (GBN93_18185)	7575..8132	+	558	WP_234966657.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8543	8543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (29-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11365.97 Da
• Isoelectric Point: 9.4144

>T141236 WP_234966663.1 NZ_CP045107:c3177-2878 [Acinetobacter johnsonii]
IFTKQIKELVSDEEYRQLQQDLLVQPDKGDVVKNGGGIRKVRCAQGNKGKSGGIRVTYYWVNEDHQIFFLVAYPKSVKDN
LTDKETAILRQLVKEQFHG

Nucleotide

• Length: 300 bp

>T141236 NZ_CP059900:c4154077-4153736 [Tomitella sp. HY172]
GTGGTGATCCGCCGCGGCGCGCTGTACTGGGCCGATGTGGGCCCCGCCGTCGGCAGTCGGCCCGCCGAGCGCCGCCCGGT
GCTTGTGATCCAGGCCAATCCGTACAACGACAGCCGCCTCGCCACGGCGGTCGCCGCCGTCGTCACCTCGAACACCGCCC
TCGCCGCGATGCCCGGCAACGTGTTCCTGCCCGCAGCCGTCACCGGGCTGCCCCGTGATTCGGTGGTCAACGTGACTGCG
CTGGTCACTCTGGACAAGGGGGACCTGACCGAGCAGGCGGGCGTCGTGCCGGCGGCGATCCTGGATGAGGTGGACCGCGG
CCTGCGCCGGCTGGGCCTGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10171.61 Da
• Isoelectric Point: 8.6582

>AT141236 WP_234966661.1 NZ_CP045107:c2885-2613 [Acinetobacter johnsonii]
MDNNLFDDLVTSIKEAGAIKRKEVKASRITELEFPDIKEVREKTGLSQNEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT141236 NZ_CP059900:c4154340-4154071 [Tomitella sp. HY172]
ATGAAGACGGCGATCTCCCTGCCCGACGACACCTACGACCGCGCCACCCGCCGCGCCGGCGAGCTGGGGCTGAGCCGGTC
GGAGTTCTTCGCCCGCGCCGCGCTGCGCTACCTCGACGAGCTGGACGCACGTTCGCTCACCGCCCGGATCGACGAGGCGC
TCGACACCGTCGCCGCGGCGGGCGCTGGGGACGCAGAGGGTGATTCGGGCTCGGGCGATGCCATCGCGGCCGGTCGCGCG
GTGCTCGGCGCCCTGGACGACGAGTGGTGA