Detailed information of TA system TA141235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2212..2863
• Replicon: plasmid pAJ4132
• Accession: NZ_CP045105
• Organism: Acinetobacter johnsonii strain AJ413

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GBN93_RS18005
• Protein ID: WP_234966624.1
• Coordinates: 2507..2863 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBN93_RS18000
• Protein ID: WP_234966622.1
• Coordinates: 2212..2514 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN93_RS17990 (GBN93_18035)	479..1240	-	762	WP_234966618.1	serine/threonine-protein phosphatase	-
GBN93_RS17995 (GBN93_18040)	1228..1989	-	762	WP_234966620.1	serine/threonine-protein kinase	-
GBN93_RS18000 (GBN93_18045)	2212..2514	-	303	WP_234966622.1	XRE family transcriptional regulator	Antitoxin
GBN93_RS18005 (GBN93_18050)	2507..2863	-	357	WP_234966624.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBN93_RS18010 (GBN93_18055)	3494..5488	+	1995	WP_234966626.1	YadA C-terminal domain-containing protein	-
GBN93_RS18015 (GBN93_18060)	5699..6229	-	531	WP_234966608.1	plasmid replication DNA-binding protein	-
GBN93_RS18020 (GBN93_18065)	6308..7243	-	936	WP_234966610.1	replication initiation protein RepM	-
GBN93_RS18025	7372..7530	-	159	WP_234966612.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10689	10689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13612.65 Da
• Isoelectric Point: 7.1651

>T141235 WP_234966624.1 NZ_CP045105:c2863-2507 [Acinetobacter johnsonii]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTILGDKSNG

Nucleotide

• Length: 357 bp

>T141235 NZ_CP059900:c3838882-3838631 [Tomitella sp. HY172]
GTGCGGACGATCTCTTATTCGGAATCACGGGCGCGGTACGCGGAAACGCTCGATAGCGTCATCGACGATCGCGAGGAGGT
CATCGTCACGCGTGCGGGACGCGACCCGGTGGTGATGGTGGCGCTCGACGAGTACGAGTCCCTCAAGGAGACGGCGTACC
TGCTCAAGAGTCCCGAGAATGCGCGTCGCCTCCTCGCCTCCATCAAGCGACTCGAATCGGGGGAAGGGGTCGAGCGGGAT
CTCGCCGAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11268.03 Da
• Isoelectric Point: 5.1991

>AT141235 WP_234966622.1 NZ_CP045105:c2514-2212 [Acinetobacter johnsonii]
MAKTLQELLASRSPESQARIQQMADELLLETQLYLIREELEISQRELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT141235 NZ_CP059900:c3838634-3838374 [Tomitella sp. HY172]
ATGAGGCTGGTCTGGGACGAGGCGGCGTGGGACGACTACCTGTGGTGGCAGGCGCAGGACCGCAAGACGCTCAAGCGCAT
CAACGCGCTGATCCGCGATATCCAGCGCAACGGCAATGAGGGCATCGGGAAGCCCGAGCAGCTCAGACACGGATTCCAGG
GCTACTGGTCGCGGCGGATCACCCTGGAGCATCGGTTGGTGTACAAAATCATCGGCGACGAGATCCGGTTCGCCGCATGC
CGATACCACTACGGGGAGTGA