Detailed information of TA system TA141231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2601731..2602382
• Replicon: chromosome
• Accession: NZ_CP045101
• Organism: Acinetobacter johnsonii strain AJ210

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GBN68_RS12655
• Protein ID: WP_183176160.1
• Coordinates: 2602026..2602382 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBN68_RS12650
• Protein ID: WP_001140620.1
• Coordinates: 2601731..2602033 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN68_RS12620 (GBN68_12650)	2598282..2598512	-	231	Protein_2445	FCD domain-containing protein	-
GBN68_RS12625 (GBN68_12655)	2598585..2598827	-	243	Protein_2446	integrase core domain-containing protein	-
GBN68_RS12630 (GBN68_12660)	2598904..2599608	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
GBN68_RS12635 (GBN68_12665)	2599672..2600952	-	1281	WP_005093338.1	cation transporter	-
GBN68_RS12640 (GBN68_12670)	2601042..2601434	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
GBN68_RS12645 (GBN68_12675)	2601528..2601689	+	162	Protein_2450	IS6 family transposase	-
GBN68_RS12650 (GBN68_12680)	2601731..2602033	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
GBN68_RS12655 (GBN68_12685)	2602026..2602382	-	357	WP_183176160.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBN68_RS12660 (GBN68_12690)	2602903..2603874	-	972	WP_004733159.1	helix-turn-helix domain-containing protein	-
GBN68_RS12665 (GBN68_12695)	2604007..2604891	+	885	WP_004733158.1	MBL fold metallo-hydrolase	-
GBN68_RS12670 (GBN68_12700)	2604976..2605518	+	543	WP_183176162.1	cysteine hydrolase family protein	-
GBN68_RS12675 (GBN68_12705)	2605711..2605911	+	201	Protein_2456	recombinase family protein	-
GBN68_RS12680 (GBN68_12710)	2605960..2606598	-	639	WP_234954062.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2598585..2607570	8985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13615.57 Da
• Isoelectric Point: 7.1651

>T141231 WP_183176160.1 NZ_CP045101:c2602382-2602026 [Acinetobacter johnsonii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRSLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTQGDKSNG

Nucleotide

• Length: 357 bp

>T141231 NZ_CP059900:1834603-1835028 [Tomitella sp. HY172]
GTGATCATTCTCGACACGAACGTGATCTCGGAGATCTTTCGACCGCTGCCTGAGCCACGCGTGGTGGACTGGCTCGCGTC
CCTGGAGGGGGACGTCGCGATTACGTCCGTCACGCTCGCGGAGCTCTTGGCGGGTGTGCGACGGTTACCGGATGGTCAGC
GCAGGGGTGAGCTGACCAGGCGCATCAACGCGGCGCTCGCACCGTACCGGGGCGGCCGCGCTGTGCTGCCGTTCGATGAC
GTGGCGGCCGACCGCTACGCCGACGTGCTCGTGGCTCGAGAGAGCACGGGAGCGCCGATCAGTACCGCCGACGCGCAGAT
CGCCGCGATCTGCCTGACGCACGGAGCAACCTGCGCTACGCGCAATGTGCAGGACTTCCAGCACACCGGCGTCGAACTGG
TGGACCCGTGGAAGGTCGACGCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT141231 WP_001140620.1 NZ_CP045101:c2602033-2601731 [Acinetobacter johnsonii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT141231 NZ_CP059900:1834373-1834606 [Tomitella sp. HY172]
ATGTCATCCATCATCGTTCGCGGTCTCGACGACGCCGTGAAACAGCAACTCGCCGCGCAGGCGAAGGAGCACGGTCGGTC
CATGGAAGCCGAGGTTCGCGCCATCCTTACCAAGGCTGCACGCAGGCCGCACATCGGAATCGCTCTGCTGGCTGCCGCGC
AGGATGTCGGTGGAGTCGAAGATCTGCCGATTCCGGTGCGCGATGACGTCGCGCGGGCGGTGGACTTCGAGTGA