Detailed information of TA system TA141206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1363812..1364536
• Replicon: chromosome
• Accession: NZ_CP045097
• Organism: Pectobacterium carotovorum strain RC5297

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: F9W95_RS06090
• Protein ID: WP_039482109.1
• Coordinates: 1363812..1364123 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F9W95_RS06095
• Protein ID: WP_103861592.1
• Coordinates: 1364120..1364536 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9W95_RS06070 (F9W95_06100)	1359702..1360436	+	735	WP_103861595.1	type I-E CRISPR-associated protein Cas5/CasD	-
F9W95_RS06075 (F9W95_06105)	1360436..1361083	+	648	WP_130637133.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
F9W95_RS06080 (F9W95_06110)	1361111..1362028	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1e	-
F9W95_RS06085 (F9W95_06115)	1362029..1362322	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
F9W95_RS06090 (F9W95_06120)	1363812..1364123	+	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
F9W95_RS06095 (F9W95_06125)	1364120..1364536	+	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
F9W95_RS06100 (F9W95_06130)	1364756..1365259	-	504	WP_109224920.1	hypothetical protein	-
F9W95_RS06105 (F9W95_06135)	1365513..1366538	-	1026	WP_120358785.1	IS630 family transposase	-
F9W95_RS06110 (F9W95_06140)	1366707..1366943	+	237	WP_233960069.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
F9W95_RS06115 (F9W95_06145)	1367465..1367923	+	459	WP_039470406.1	hypothetical protein	-
F9W95_RS06120 (F9W95_06150)	1368021..1369373	-	1353	WP_240004624.1	pyridoxal-dependent decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1365513..1366538	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T141206 WP_039482109.1 NZ_CP045097:1363812-1364123 [Pectobacterium carotovorum]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T141206 NZ_CP059889:851224-851634 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT141206 WP_103861592.1 NZ_CP045097:1364120-1364536 [Pectobacterium carotovorum]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT141206 NZ_CP059889:850977-851243 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A093VC08