Detailed information of TA system TA141128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 94985..95499
• Replicon: chromosome
• Accession: NZ_CP045034
• Organism: Limosilactobacillus fermentum strain USM 8633

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6C1EMT8
• Locus tag: A0J18_RS00470
• Protein ID: WP_035437199.1
• Coordinates: 94985..95248 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: A0J18_RS00475
• Protein ID: WP_035437201.1
• Coordinates: 95245..95499 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0J18_RS00455	90461..91243	+	783	WP_035437193.1	dimethylargininase	-
A0J18_RS00460	91631..93028	+	1398	WP_035437196.1	sodium:proton antiporter	-
A0J18_RS00465	93454..94575	+	1122	WP_080650628.1	PTS sugar transporter subunit IIC	-
A0J18_RS00470	94985..95248	-	264	WP_035437199.1	Txe/YoeB family addiction module toxin	Toxin
A0J18_RS00475	95245..95499	-	255	WP_035437201.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A0J18_RS00480	95748..96035	+	288	WP_041807836.1	transposase	-
A0J18_RS00485	96059..96937	+	879	WP_086031809.1	IS3 family transposase	-
A0J18_RS00490	97735..98499	+	765	WP_080650602.1	zinc ribbon domain-containing protein	-
A0J18_RS00495	98706..99611	-	906	WP_154244344.1	IS3 family transposase	-
A0J18_RS00500	99548..100162	-	615	WP_035431426.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-78)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10252.94 Da
• Isoelectric Point: 10.3349

>T141128 WP_035437199.1 NZ_CP045034:c95248-94985 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVLPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHDE

Nucleotide

• Length: 264 bp

>T141128 NZ_CP059835:2198367-2198744 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9773.05 Da
• Isoelectric Point: 4.4237

>AT141128 WP_035437201.1 NZ_CP045034:c95499-95245 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTRADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT141128 NZ_CP059835:2197909-2198277 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A6C1EMT8