Detailed information of TA system TA141121

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22892..23161
• Replicon: plasmid pBK13048-6
• Accession: NZ_CP045020
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain BK13048

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F9865_RS27455
• Protein ID: WP_001312861.1
• Coordinates: 23003..23161 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22892..22957 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9865_RS27430	18602..19129	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
F9865_RS27435	19187..19420	+	234	WP_000006003.1	DUF905 family protein	-
F9865_RS27440	19481..21504	+	2024	Protein_25	ParB/RepB/Spo0J family partition protein	-
F9865_RS27445	21573..22007	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F9865_RS27450	22004..22723	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	22735..22959	+	225	NuclAT_0	-	-
-	22735..22959	+	225	NuclAT_0	-	-
-	22735..22959	+	225	NuclAT_0	-	-
-	22735..22959	+	225	NuclAT_0	-	-
-	22892..22957	+	66	-	-	Antitoxin
F9865_RS27455	23003..23161	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
F9865_RS27460	23519..23944	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
F9865_RS27465	23941..24291	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
F9865_RS27470	24322..25935	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
F9865_RS28400	26020..26224	-	205	Protein_32	pilus protein	-
F9865_RS27490	26671..27368	+	698	Protein_33	IS1 family transposase	-
F9865_RS27495	27365..27931	+	567	WP_151887008.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28729	28729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T141121 WP_001312861.1 NZ_CP045020:23003-23161 [Klebsiella pneumoniae subsp. pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T141121 NZ_CP059835:184181-184357 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT141121 NZ_CP045020:22892-22957 [Klebsiella pneumoniae subsp. pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin