Detailed information of TA system TA141103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 353239..353879
• Replicon: chromosome
• Accession: NZ_CP045015
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain BK13048

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: F9865_RS01650
• Protein ID: WP_016529833.1
• Coordinates: 353239..353586 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F9865_RS01655
• Protein ID: WP_040181955.1
• Coordinates: 353586..353879 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9865_RS01640	349165..350598	+	1434	WP_002920564.1	glycogen synthase GlgA	-
F9865_RS01645	350616..353063	+	2448	WP_002920561.1	glycogen phosphorylase	-
F9865_RS01650	353239..353586	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F9865_RS01655	353586..353879	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
F9865_RS01660	353949..355457	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
F9865_RS01665	355662..355991	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
F9865_RS01670	356042..356872	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
F9865_RS01675	356922..357680	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T141103 WP_016529833.1 NZ_CP045015:353239-353586 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T141103 NZ_CP059794:18472-18729 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT141103 WP_040181955.1 NZ_CP045015:353586-353879 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT141103 NZ_CP059794:18210-18479 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: W9BBY1