Detailed information of TA system TA141090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5653926..5654521
• Replicon: chromosome
• Accession: NZ_CP045002
• Organism: Pseudomonas aeruginosa strain PAG5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: F9C43_RS26390
• Protein ID: WP_003113526.1
• Coordinates: 5654243..5654521 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F9C43_RS26385
• Protein ID: WP_003099268.1
• Coordinates: 5653926..5654231 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9C43_RS26350	5649277..5649549	-	273	WP_003115921.1	hypothetical protein	-
F9C43_RS26355	5649996..5650331	+	336	WP_116802237.1	hypothetical protein	-
F9C43_RS26360	5650377..5651972	-	1596	WP_147314246.1	DUF2326 domain-containing protein	-
F9C43_RS26365	5651962..5652201	-	240	WP_165567201.1	hypothetical protein	-
F9C43_RS26370	5652201..5652695	-	495	WP_147314247.1	hypothetical protein	-
F9C43_RS26375	5652695..5652871	-	177	WP_165567202.1	hypothetical protein	-
F9C43_RS26380	5653212..5653589	-	378	WP_116802239.1	hypothetical protein	-
F9C43_RS26385	5653926..5654231	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
F9C43_RS26390	5654243..5654521	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F9C43_RS26395	5654850..5657078	+	2229	WP_003113525.1	TonB-dependent receptor	-
F9C43_RS26400	5657148..5657795	-	648	WP_003095021.1	carbonate dehydratase	-
F9C43_RS26405	5657857..5659095	-	1239	WP_003123433.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T141090 WP_003113526.1 NZ_CP045002:c5654521-5654243 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T141090 NZ_CP059765:c19186-18323 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT141090 WP_003099268.1 NZ_CP045002:c5654231-5653926 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT141090 NZ_CP059765:c19460-19188 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3