Detailed information of TA system TA141039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2232..2883
• Replicon: plasmid p29FS20-2
• Accession: NZ_CP044521
• Organism: Acinetobacter baumannii strain 29FS20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A335S9M6
• Locus tag: F7R60_RS19815
• Protein ID: WP_074163944.1
• Coordinates: 2527..2883 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7R60_RS19810
• Protein ID: WP_001140621.1
• Coordinates: 2232..2534 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7R60_RS19785	40..111	-	72	Protein_0	type II toxin-antitoxin system HipA family toxin	-
F7R60_RS19790	201..668	+	468	WP_114242002.1	DUF1643 domain-containing protein	-
F7R60_RS19795	786..1298	-	513	WP_001089572.1	SMI1/KNR4 family protein	-
F7R60_RS19800	1337..1855	-	519	WP_024437861.1	sel1 repeat family protein	-
F7R60_RS19805	2030..2239	-	210	WP_000069474.1	hypothetical protein	-
F7R60_RS19810	2232..2534	-	303	WP_001140621.1	XRE family transcriptional regulator	Antitoxin
F7R60_RS19815	2527..2883	-	357	WP_074163944.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7R60_RS19820	3470..4111	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
F7R60_RS19825	4177..5364	+	1188	WP_065996278.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
F7R60_RS19830	5581..6105	-	525	WP_005127114.1	plasmid replication DNA-binding protein	-
F7R60_RS19835	6184..7113	-	930	WP_005133531.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / aph(6)-Id / aph(3'')-Ib / sul2	-	1..12731	12731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13529.52 Da
• Isoelectric Point: 6.4631

>T141039 WP_074163944.1 NZ_CP044521:c2883-2527 [Acinetobacter baumannii]
MWTIITTDLFNEWLVQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T141039 NZ_CP059723:c2000741-2000406 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.02 Da
• Isoelectric Point: 7.3198

>AT141039 WP_001140621.1 NZ_CP044521:c2534-2232 [Acinetobacter baumannii]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT141039 NZ_CP059723:c2000989-2000741 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A335S9M6