Detailed information of TA system TA141007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 36158..36743
• Replicon: plasmid pHZE30-1-1
• Accession: NZ_CP044484
• Organism: Acinetobacter schindleri strain HZE30-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: FSC11_RS15005
• Protein ID: WP_000897307.1
• Coordinates: 36158..36478 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FSC11_RS15010
• Protein ID: WP_000369781.1
• Coordinates: 36471..36743 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC11_RS14965	31368..31787	-	420	WP_163167820.1	hypothetical protein	-
FSC11_RS14970	32093..32380	+	288	WP_000438825.1	BrnT family toxin	-
FSC11_RS14975	32367..32681	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FSC11_RS14980	32770..33300	-	531	WP_000172759.1	hypothetical protein	-
FSC11_RS14985	33449..33751	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FSC11_RS14990	33752..34033	+	282	WP_000985609.1	putative addiction module antidote protein	-
FSC11_RS14995	34113..34532	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FSC11_RS15000	34695..36029	+	1335	WP_015060273.1	ISNCY family transposase	-
FSC11_RS15005	36158..36478	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSC11_RS15010	36471..36743	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
FSC11_RS15020	38013..38828	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
FSC11_RS15025	38915..39157	+	243	Protein_53	phosphoglucosamine mutase	-
FSC11_RS15035	39393..40223	-	831	Protein_54	IS91-like element ISVsa3 family transposase	-
FSC11_RS15040	40287..40991	+	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
FSC11_RS15045	41045..41368	-	324	Protein_56	ATP-dependent Lon protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / floR / tet(X3) / tet(X6)	-	1..110146	110146
	-	inside	IScluster/Tn	blaNDM-1 / sul2	-	9493..40991	31498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T141007 WP_000897307.1 NZ_CP044484:36158-36478 [Acinetobacter schindleri]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T141007 NZ_CP059675:1426428-1426718 [Schaalia sp. JY-X169]
GTGAGCCGGTATCGTCTCACTCCGGCCGCGCAACGGGACCTTTCGTCGATTTGGGACTTCACGCAGGGACGCTGGGATGC
GCGTCAAGCCGAGACCTACGTTACTGAGATAAGAGCGGCACTCGAGCGCATCGCTGACAACCCAGGCCGTGGTCGCACCT
GCGATGAGATCCGCGTTGGCTACCGCAGATACTCCATTGGGAGCCACCTCATTTTCTATGTTAAGAGGCCCGATGGAGTG
GATGTCATCCGGATCCTGCATCAGAGAATGGACCCAACACGACATCTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT141007 WP_000369781.1 NZ_CP044484:36471-36743 [Acinetobacter schindleri]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT141007 NZ_CP059675:1426198-1426431 [Schaalia sp. JY-X169]
ATGGCTCAGAACACCTCAATCAGCTTGGATGATCACTTCTCGGGCTTCCTCTCCAGGGAAGTGTCCTCGGGGCGCTATCG
GTCTGCCAGTGAGGTAGTCCGTGCAGGCCTGCGGCTACTGGAAGATCAGGAGACTCAGATGGCTGCCCTCCGCGCGGCCC
TGGTGACCGGCGAAGAGAGCGGGGCGCCAGAGGTTTTTGACTTTGAGGCCTTCATCGCGGCAAAGAAGTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3