Detailed information of TA system TA140999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 8370..8955
• Replicon: plasmid pB18-6
• Accession: NZ_CP044461
• Organism: Acinetobacter indicus strain B18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0E6
• Locus tag: FSC09_RS17615
• Protein ID: WP_002126330.1
• Coordinates: 8370..8690 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FSC09_RS17620
• Protein ID: WP_004676016.1
• Coordinates: 8683..8955 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC09_RS17590	3900..4607	-	708	WP_163146731.1	type IV secretion protein Rhs	-
FSC09_RS17595	4700..5398	+	699	WP_163146736.1	hypothetical protein	-
FSC09_RS17600	6034..7425	+	1392	WP_163146732.1	hypothetical protein	-
FSC09_RS17605	7614..7919	+	306	WP_163146733.1	hypothetical protein	-
FSC09_RS17610	7980..8180	+	201	WP_163146734.1	hypothetical protein	-
FSC09_RS17615	8370..8690	+	321	WP_002126330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSC09_RS17620	8683..8955	+	273	WP_004676016.1	helix-turn-helix domain-containing protein	Antitoxin
FSC09_RS17625	9034..9609	-	576	WP_121973990.1	hypothetical protein	-
FSC09_RS17630	9615..9965	-	351	WP_121973991.1	STAS-like domain-containing protein	-
FSC09_RS17635	9958..10950	-	993	WP_121973992.1	hypothetical protein	-
FSC09_RS17640	11393..11902	+	510	WP_046206310.1	hypothetical protein	-
FSC09_RS17645	11930..12265	+	336	WP_075175697.1	hypothetical protein	-
FSC09_RS17650	12286..12456	-	171	WP_163146735.1	hypothetical protein	-
FSC09_RS17655	12459..12833	-	375	WP_046206308.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14098	14098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12191.98 Da
• Isoelectric Point: 7.9834

>T140999 WP_002126330.1 NZ_CP044461:8370-8690 [Acinetobacter indicus]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T140999 NZ_CP059590:2733426-2733560 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10178.56 Da
• Isoelectric Point: 7.3200

>AT140999 WP_004676016.1 NZ_CP044461:8683-8955 [Acinetobacter indicus]
MDNNLFDDLVASIKEAGAIKRKEIQASRTTELELPDIKEVREKTGLSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT140999 NZ_CP059590:2733210-2733362 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: A0A3F3L0E6