Detailed information of TA system TA140998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4407..5058
• Replicon: plasmid pB18-5
• Accession: NZ_CP044460
• Organism: Acinetobacter indicus strain B18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: FSC09_RS17480
• Protein ID: WP_000269911.1
• Coordinates: 4407..4763 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FSC09_RS17485
• Protein ID: WP_001140620.1
• Coordinates: 4756..5058 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC09_RS17460	184..1161	+	978	WP_127801075.1	helix-turn-helix domain-containing protein	-
FSC09_RS17465	1293..1682	-	390	WP_004781111.1	tautomerase family protein	-
FSC09_RS17470	1894..2322	-	429	WP_000877730.1	hypothetical protein	-
FSC09_RS17475	2533..3858	+	1326	WP_163145943.1	ISNCY family transposase	-
FSC09_RS17480	4407..4763	+	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSC09_RS17485	4756..5058	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
FSC09_RS17490	5395..6699	+	1305	WP_152342556.1	IS4 family transposase	-
FSC09_RS17495	6786..9182	-	2397	WP_127801717.1	DUF1080 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..21855	21855
	-	flank	IS/Tn	-	-	2521..3858	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T140998 WP_000269911.1 NZ_CP044460:4407-4763 [Acinetobacter indicus]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T140998 NZ_CP059590:2733210-2733362 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT140998 WP_001140620.1 NZ_CP044460:4756-5058 [Acinetobacter indicus]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT140998 NZ_CP059590:2733426-2733560 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3