Detailed information of TA system TA140981

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrH-as-bsrG/-
• Location: 3533132..3533366
• Replicon: chromosome
• Accession: NZ_CP044444
• Organism: Bacillus amyloliquefaciens strain KC41

Toxin (Protein)

• Gene name: bsrH
• Uniprot ID: -
• Locus tag: F7V86_RS17835
• Protein ID: WP_104843506.1
• Coordinates: 3533132..3533269 (+)
• Length: 46 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	3533216..3533366 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7V86_RS17805	3528858..3529550	+	693	WP_065180794.1	hypothetical protein	-
F7V86_RS17810	3529617..3530636	+	1020	WP_065180795.1	hypothetical protein	-
F7V86_RS17820	3530800..3532047	+	1248	WP_031600262.1	IS256-like element ISBsu2 family transposase	-
F7V86_RS17825	3532075..3532434	-	360	WP_021493945.1	hypothetical protein	-
F7V86_RS17830	3532440..3532907	-	468	WP_063636675.1	DUF4879 domain-containing protein	-
F7V86_RS17835	3533132..3533269	+	138	WP_104843506.1	putative holin-like toxin	Toxin
-	3533216..3533366	-	151	NuclAT_0	-	Antitoxin
-	3533216..3533366	-	151	NuclAT_0	-	Antitoxin
-	3533216..3533366	-	151	NuclAT_0	-	Antitoxin
-	3533216..3533366	-	151	NuclAT_0	-	Antitoxin
F7V86_RS17840	3533456..3533941	-	486	WP_065180785.1	SMI1/KNR4 family protein	-
F7V86_RS17845	3533967..3534425	-	459	WP_065180786.1	SMI1/KNR4 family protein	-
F7V86_RS17850	3534438..3536324	-	1887	WP_065180787.1	HNH endonuclease	-
F7V86_RS17855	3536361..3536798	-	438	WP_076983291.1	SMI1/KNR4 family protein	-
F7V86_RS17860	3537054..3538037	+	984	WP_065180788.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3514867..3564580	49713
	-	flank	IS/Tn	-	-	3530809..3532047	1238
	-	flank	IS/Tn	-	-	3538271..3539509	1238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-29)

Sequences

Toxin

Protein

• Length: 46 a.a.
• Molecular weight: 5096.15 Da
• Isoelectric Point: 8.6040

>T140981 WP_104843506.1 NZ_CP044444:3533132-3533269 [Bacillus amyloliquefaciens]
MLMSTFQALMLMLAFGSFIIALLTYINKKQTSLETGILKGQVYHE

Nucleotide

• Length: 138 bp

>T140981 NZ_CP059587:3906004-3906333 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin

Nucleotide

• Length: 151 bp

>AT140981 NZ_CP044444:c3533366-3533216 [Bacillus amyloliquefaciens]
TTTCTTTAATAAGAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCACCCCGGCTCTGTACAAAAAGCTACCCATCA
AAGGGCTTGCTCAGCAGTTATTCGTGATAGACCTGTCCCTTCAGGATTCCAGTCTCAAGGGAGGTCTGTTT

Structures

Antitoxin