Detailed information of TA system TA140973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3622716..3623410
• Replicon: chromosome
• Accession: NZ_CP044443
• Organism: Escherichia coli strain HeN100

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HeN100_RS19465
• Protein ID: WP_001263493.1
• Coordinates: 3622716..3623114 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HeN100_RS19470
• Protein ID: WP_000554757.1
• Coordinates: 3623117..3623410 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3618376)	3618376..3618456	-	81	NuclAT_10	-	-
- (3618376)	3618376..3618456	-	81	NuclAT_10	-	-
- (3618376)	3618376..3618456	-	81	NuclAT_10	-	-
- (3618376)	3618376..3618456	-	81	NuclAT_10	-	-
HeN100_RS19435 (3617716)	3617716..3618960	-	1245	WP_000189541.1	esterase FrsA	-
HeN100_RS19440 (3619052)	3619052..3619510	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HeN100_RS19445 (3619771)	3619771..3621228	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HeN100_RS19450 (3621285)	3621285..3621806	-	522	Protein_3477	peptide chain release factor H	-
HeN100_RS19455 (3621805)	3621805..3622008	-	204	Protein_3478	RtcB family protein	-
HeN100_RS19460 (3622254)	3622254..3622706	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HeN100_RS19465 (3622716)	3622716..3623114	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HeN100_RS19470 (3623117)	3623117..3623410	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HeN100_RS19475 (3623462)	3623462..3624517	-	1056	WP_001226164.1	DNA polymerase IV	-
HeN100_RS19480 (3624588)	3624588..3625373	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HeN100_RS19485 (3625345)	3625345..3627057	+	1713	Protein_3484	flagellar biosynthesis protein FlhA	-
HeN100_RS19490 (3627273)	3627273..3627770	-	498	WP_000006256.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T140973 WP_001263493.1 NZ_CP044443:c3623114-3622716 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140973 NZ_CP059586:1548874-1549797 [Xanthomonas oryzae pv. oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACTGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT140973 WP_000554757.1 NZ_CP044443:c3623410-3623117 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140973 NZ_CP059586:1548278-1548877 [Xanthomonas oryzae pv. oryzae]
ATGGACACGACCGTCGACAAGCTGCTGGAGCTGGTGCGTACGCGGGGACTTCTGCGCCCGCACGATCTGGCCCCACTGGG
CATCCCACGGGTGGCCCTGACGCGGGCCGTTCGGCGCGGGCAACTGGAGCGCGTCGGGCGCGGCCTGTACGGCCTGGTGG
CGCGTCCGGTGTCGGCCCACGGCACGCTGGCCGAAGTCGCGCGCCGCGTGCCCAAGGGCGTGGTCTGCCTGCTGTCGGCG
CTGCGCGTCCACGACCTGACGACCCAGGCCCCGTTCGAGGTGTGGCTGGCCATCGACAACAAGGCCGCTGCCCCGAAGCT
GGACTACCCGCCGCTACGTCTCGTGCGCTTCTCCGGGCCGGCGCTGAGCGAGGGCGTCGAGGAACACGTTGTCGATGGTG
TGCCCGTCCACGTGACCAGCGTCGCCAAGACCGTGGCCGACTGCTTCAAGTACCGCAACAAGATCGGCATCGACGTGGCG
CTGGAAGCGCTGCGCGAGGCGTGGGTTGCCCGGCGCATGACCAGCGACGAGATCTGGCACTACGCCAAGATCGACCGCGT
CGCCAACGTGATGCGTCCCTATCTGGAGAGCCTGGCATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6