Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 641999..642726 Replicon chromosome
Accession NZ_CP044443
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HeN100_RS04890 Protein ID WP_000550189.1
Coordinates 641999..642313 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HeN100_RS04895 Protein ID WP_000560266.1
Coordinates 642310..642726 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS04870 (638157) 638157..639143 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HeN100_RS04875 (639222) 639222..639914 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HeN100_RS04880 (639991) 639991..640494 - 504 WP_001300832.1 M48 family metallopeptidase -
HeN100_RS04885 (640579) 640579..641715 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HeN100_RS04890 (641999) 641999..642313 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HeN100_RS04895 (642310) 642310..642726 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HeN100_RS04900 (642771) 642771..644789 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HeN100_RS04905 (645215) 645215..647566 - 2352 WP_000695489.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140957 WP_000550189.1 NZ_CP044443:641999-642313 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140957 NZ_CP059583:420888-421022 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT140957 WP_000560266.1 NZ_CP044443:642310-642726 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140957 NZ_CP059583:420672-420824 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References