Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3982961..3983760 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FNW43_RS19285 Protein ID WP_000347273.1
Coordinates 3983296..3983760 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FNW43_RS19280 Protein ID WP_001307405.1
Coordinates 3982961..3983296 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS19265 3978746..3979516 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
FNW43_RS19270 3979532..3980866 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FNW43_RS19275 3981241..3982812 + 1572 WP_001273753.1 galactarate dehydratase -
FNW43_RS19280 3982961..3983296 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FNW43_RS19285 3983296..3983760 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FNW43_RS19290 3983815..3984624 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FNW43_RS19295 3984873..3986153 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FNW43_RS19300 3986176..3986649 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FNW43_RS19305 3986660..3987031 + 372 Protein_3726 PTS sugar transporter subunit IIC -
FNW43_RS19310 3987027..3987584 + 558 Protein_3727 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3973814..3994744 20930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T140910 WP_000347273.1 NZ_CP044410:3983296-3983760 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T140910 NZ_CP059560:c5121290-5121024 [Aromatoleum petrolei]
ATGGAGAAACGAACCCCGCACTGCAAGCTGGCAGTGGTCAAAGCTTTGGTCGAGGCGGGTATGGTGCGCACGACCCACGC
GGCGCGAACTGGCGCGAACGAGTTGGGGCTCGAGTTTTCCGACATGCTGGCCGTGGTGATGGCACTCACGCCAGCGGACT
TCTACAAGAGCATGACCACCCACGCCGATCATACGCTCTGGCAGGACGTGTACCGCCCAAACACGCGGGCGGGCGGGCGA
CGTGTACCTGAAGCTGACAGTCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140910 WP_001307405.1 NZ_CP044410:3982961-3983296 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140910 NZ_CP059560:c5120987-5120586 [Aromatoleum petrolei]
ATGAAATGTCCTGTGTGCGGCGCGGCGGAACTGATCCACGACACCCGCGACCTGCCCTACACCTACAAGGGCGAAACCAC
CACCATTGTGGCGGTGACGGGCGACTTCTGCCCGGCCTGCGCCGAGTCGGTCCTGGACGCAGCGGAATCGGATCGCGTCA
TGCGCGAAATGCGCGCCTTCTCGAAGCAGGTGAATGCGGCCATTGTCGATCCCAGCTTCATCGCCAACGTGCGCAAGAAG
CTCGCGCTTGACCAGCGCGAAGCCGCCGAAATCTTTGGCGGCGGTATCAACGCCTTCTCGCGCTACGAGAACGGCAAGAC
CAAGCCGCCGCTGGCCCTGGTGAAACTGCTCAAGGTACTGGATCGTCATCCCGACCTGCTCAACGAGGTCAGAACCGCCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References