Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3939688..3940415 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag FNW43_RS19065 Protein ID WP_000550189.1
Coordinates 3940101..3940415 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FNW43_RS19060 Protein ID WP_000560266.1
Coordinates 3939688..3940104 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS19050 3934848..3937199 + 2352 WP_000695487.1 alpha-glucosidase -
FNW43_RS19055 3937625..3939643 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
FNW43_RS19060 3939688..3940104 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FNW43_RS19065 3940101..3940415 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
FNW43_RS19070 3940699..3941835 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
FNW43_RS19075 3941920..3942423 + 504 WP_001333820.1 M48 family metallopeptidase -
FNW43_RS19080 3942500..3943192 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
FNW43_RS19085 3943271..3944257 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140909 WP_000550189.1 NZ_CP044410:c3940415-3940101 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140909 NZ_CP059560:c424553-424263 [Aromatoleum petrolei]
ATGAAGACCACTCTTACCACCGAGTTCTTCGACGCTTGGTTTGCCTGTCTCCGGGACCCGCAGGCAGCGGCCCGCATCAA
GATGCGGATCGGCCGGGCAGAGTTGGGCGACTTCGGCGACTGTGAATCGGTGGGAGAGGGGGTTTCAGAGATGCGGATCC
ATTATGGGCCGGGCTACCGCGTGTATTTCACCCAGCGCGGCATGGAAGTGGTGATCCTGCTGGCGGGCGGCGACAAGACC
ACCCAAGCCAAGGACATCAAGACCGCCTTGAAGTTGGCACGGGAACTGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT140909 WP_000560266.1 NZ_CP044410:c3940104-3939688 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140909 NZ_CP059560:c424253-423951 [Aromatoleum petrolei]
ATGGCACTCAAGCTGCGAAAATGGAACACGGCGGAGCACCTCAAGACCGAGGAGGAAATGGCGCTCTACCTCGAGGCTTG
CCTGCAAGAAGCCGGGGACGATGCGGCCTTCATCGCCAAGGCACTGGGCGATATTGCCCGCGCCAAAGGCATGACCCAAC
TGGCCAAGGACACCGGTCTGGGGCGGGAGAGCCTGTACAAGGCCCTTTCCGGCGAGGGCAATCCGAGCTTTGCCACCATC
CTCAAGGTGACGCACGCGTTGGGCCTGCAACTGCACGCGGCACCACGCCACGCGCCGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References