Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 959370..960064 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FNW43_RS04485 Protein ID WP_001263489.1
Coordinates 959666..960064 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FNW43_RS04480 Protein ID WP_000554758.1
Coordinates 959370..959663 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS04460 955002..955499 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FNW43_RS04465 955723..957435 - 1713 Protein_849 flagellar biosynthesis protein FlhA -
FNW43_RS04470 957407..958192 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FNW43_RS04475 958263..959318 + 1056 WP_001226164.1 DNA polymerase IV -
FNW43_RS04480 959370..959663 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FNW43_RS04485 959666..960064 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FNW43_RS04490 960074..960526 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FNW43_RS04495 960844..961050 + 207 Protein_855 RtcB family protein -
FNW43_RS04500 961046..961567 + 522 Protein_856 peptide chain release factor H -
FNW43_RS04505 961624..963081 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FNW43_RS04510 963342..963800 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 964396..964476 + 81 NuclAT_12 - -
- 964396..964476 + 81 NuclAT_12 - -
- 964396..964476 + 81 NuclAT_12 - -
- 964396..964476 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T140878 WP_001263489.1 NZ_CP044410:959666-960064 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T140878 NZ_CP059488:2478303-2478638 [Acidithiobacillus ferrivorans]
GTGAACGACGCGCGGCGTGGTGATTTGTTGACGGTGGCTATACGGGGTGACTTCGGGAAACCAAGACCGGCGCTGGTGGT
GCAGTCTGATCAGTTTAGCGAGCAGGCGACCGTCACCGTGTTACTACTGTCGGGAACGCTAGTGGATGCGCCATTGTTCC
GGATAACAGTACATCCTGACGGGACCAATGGATTACAAAAGACTTCACAGATCATGGTCGACAAGGTAATGACGGTAAAG
CGCGATAAGTTGGGCCCCGTATTTGGTTCCGTTAGCGCGCAGGTGATAATAGAGGTGAGTCGTTGCCTGGCGGTGTTTTT
AGGTATCGCAAAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT140878 WP_000554758.1 NZ_CP044410:959370-959663 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT140878 NZ_CP059488:2478091-2478306 [Acidithiobacillus ferrivorans]
ATGATGACCACCCACGTGAATGAGCGCGTCCAAAAGTGCCGCAATGCTCTGCGCAGAGCTGGCCTCCGTCCAGTTCAAAT
CTGGGTGCCAGACACGCGCCGCCCCGGATTTGCAGAAGAGTGCCATCGCCAATCCCGATTAGCTGCCCAAGCAGATAGGG
CGGATACGGATCTGATGGAGTTTCTGGATGACGCACTGGATGATTTGGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References