Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 953326..953867 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FNW43_RS04445 Protein ID WP_000615983.1
Coordinates 953326..953604 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FNW43_RS04450 Protein ID WP_000729703.1
Coordinates 953607..953867 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS04430 950908..951486 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FNW43_RS04435 951692..952459 + 768 WP_000333380.1 class II glutamine amidotransferase -
FNW43_RS04440 952430..953170 - 741 WP_001225679.1 murein L,D-transpeptidase -
FNW43_RS04445 953326..953604 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNW43_RS04450 953607..953867 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNW43_RS04455 954077..954826 + 750 WP_000056849.1 C40 family peptidase -
FNW43_RS04460 955002..955499 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FNW43_RS04465 955723..957435 - 1713 Protein_849 flagellar biosynthesis protein FlhA -
FNW43_RS04470 957407..958192 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T140877 WP_000615983.1 NZ_CP044410:c953604-953326 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T140877 NZ_CP059488:c2424488-2424264 [Acidithiobacillus ferrivorans]
ATGGGCAATATTCCCGTTCTCAAGCCCAATGAAGTAATCGCTATTCTCATTAAACTCGGCTTCATGGAAGTCCGCCAACG
CGGATCACACAAACAATTCCGCGATACCGCAGGCCGCTGCACAACGGTTCCGTTTCATCAAGGTCGAGACATCTCCCCAA
TCTTGCTCCGTCAAATCGCCAAAGACATCGGCCTCGCGCTTGATGAGTTGCTCAAGCACAGATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT140877 WP_000729703.1 NZ_CP044410:c953867-953607 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT140877 NZ_CP059488:c2424701-2424492 [Acidithiobacillus ferrivorans]
ATGAAAGTCTATTCAGCGGTAATTGAGCGGTGCCCACAAACTGGCCTGTTCGTTGGTTTTGTTCCTGGTTTTCCCGGCGC
TCATTCCCAGGGGGAAACGCTGGATGAAGTCAACCACAACCTGCACGATGTTATTGCAATGCTTCTGGAAGATGGCGAAC
CCATGCTGGAGTCCGAGTTTGTCGGGGTTCAAAACGTCGCCATCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References