Detailed information of TA system TA140862

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80406..80827
• Replicon: plasmid pNMBU-W10C18_01
• Accession: NZ_CP044404
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: F7O57_RS25500
• Protein ID: WP_001302184.1
• Coordinates: 80406..80564 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	80629..80827 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS25460	75470..75685	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
F7O57_RS25465	75821..76468	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
F7O57_RS25470	76659..77042	-	384	WP_001063020.1	relaxosome protein TraM	-
F7O57_RS25475	77412..77984	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
F7O57_RS25480	78280..79101	-	822	WP_001234465.1	DUF945 domain-containing protein	-
F7O57_RS25485	79220..79507	-	288	WP_000107242.1	hypothetical protein	-
F7O57_RS25490	79532..79738	-	207	WP_000275854.1	hypothetical protein	-
F7O57_RS25495	79740..79928	-	189	WP_032147509.1	hypothetical protein	-
F7O57_RS25500	80406..80564	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	80629..80827	-	199	NuclAT_0	-	Antitoxin
-	80629..80827	-	199	NuclAT_0	-	Antitoxin
-	80629..80827	-	199	NuclAT_0	-	Antitoxin
-	80629..80827	-	199	NuclAT_0	-	Antitoxin
F7O57_RS25505	80796..80901	-	106	Protein_91	plasmid SOS inhibition protein A	-
F7O57_RS25510	81056..81373	+	318	Protein_92	IS110 family transposase	-
F7O57_RS25515	81662..81913	-	252	Protein_93	iron-containing alcohol dehydrogenase	-
F7O57_RS25520	81998..82621	-	624	WP_000912556.1	potassium channel family protein	-
F7O57_RS25525	82637..83330	-	694	Protein_95	IS1 family transposase	-
F7O57_RS25530	83387..83491	+	105	Protein_96	thiosulfate reductase cytochrome B subunit	-
F7O57_RS25535	83737..85131	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..114329	114329
	-	flank	IS/Tn	-	-	82637..83014	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T140862 WP_001302184.1 NZ_CP044404:c80564-80406 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T140862 NZ_CP059488:c314446-314081 [Acidithiobacillus ferrivorans]
GTGGATACATCGGTGTGGGTCGATCACTTCCGGCATCGCAATGATGCGCTGGTTGAACTGCTGGAGCGTGATCTGGTGAT
GGCGCACCCTTTGGTCTTGGGCGAAATCGCCTGTGGAACACCGCCTAATCGGGACAAGACGCTGTCCGATCTCGACCGCT
TGCAGTCTACTCAACAGGCCACAGTGCGGGAGACGATGGCGTTCATTGGGCGCGAGCGACTGTTCGGTTTGGGTTGCGGC
CTGATAGACATCCTTTTGCTGGCATCCACCATGATAACGCCGGGGACGGAGCTGTGGACACTGGACAAACGGCTGTGCGC
GTTGGCGGAGCGGTTTGGCGTGAAGCACGGGTCATTCCTGCATTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT140862 NZ_CP044404:c80827-80629 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin