Detailed information of TA system TA140860

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32986..33240
• Replicon: plasmid pNMBU-W10C18_01
• Accession: NZ_CP044404
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F7O57_RS25210
• Protein ID: WP_032215358.1
• Coordinates: 32986..33192 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	33179..33240 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS25175	28009..28713	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F7O57_RS25180	29009..29410	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
F7O57_RS25185	29343..29600	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
F7O57_RS25190	29693..30346	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
F7O57_RS25195	31285..32142	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
F7O57_RS25200	32135..32209	-	75	WP_001365705.1	RepA leader peptide Tap	-
F7O57_RS25205	32445..32702	-	258	WP_000083833.1	replication regulatory protein RepA	-
F7O57_RS25210	32986..33192	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33179..33240	+	62	NuclAT_1	-	Antitoxin
-	33179..33240	+	62	NuclAT_1	-	Antitoxin
-	33179..33240	+	62	NuclAT_1	-	Antitoxin
-	33179..33240	+	62	NuclAT_1	-	Antitoxin
F7O57_RS25215	33379..33645	-	267	WP_087757657.1	hypothetical protein	-
F7O57_RS25220	34020..34589	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
F7O57_RS25225	34741..35367	-	627	WP_029702149.1	hypothetical protein	-
F7O57_RS25230	35779..35988	-	210	WP_039023209.1	hypothetical protein	-
F7O57_RS25235	36119..36679	-	561	WP_001567328.1	fertility inhibition protein FinO	-
F7O57_RS25240	36734..37480	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..114329	114329
	-	flank	IS/Tn	-	-	28009..28713	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T140860 WP_032215358.1 NZ_CP044404:c33192-32986 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T140860 NZ_CP059488:51818-52072 [Acidithiobacillus ferrivorans]
GTGAGGCTGATCTTCTCTGACGATGCATGGGAAGACTACCTATATTGGCAGAAGCAAGACAAGCGCATGGTCGAGCGAAT
CAATAAACTGATACATGAGACACAGCGTGAACCCTTCGCGGGAGTTGGCAAACCCGAGGCACTGAAGCATGCTCTTTCAG
GATTTTGGTCACGCCGTATTACGGATGAGCATCGCATGGTGTATCGAATAGAAGGTGATGCATTGATGATTGCTCAGTTA
CGTTTCCACTACTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT140860 NZ_CP044404:33179-33240 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin