Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4451396..4452195 Replicon chromosome
Accession NZ_CP044403
Organism Escherichia coli strain NMBU-W10C18

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag F7O57_RS21850 Protein ID WP_000347272.1
Coordinates 4451731..4452195 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F7O57_RS21845 Protein ID WP_001307405.1
Coordinates 4451396..4451731 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7O57_RS21830 4447181..4447951 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
F7O57_RS21835 4447967..4449301 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
F7O57_RS21840 4449676..4451247 + 1572 WP_001273738.1 galactarate dehydratase -
F7O57_RS21845 4451396..4451731 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F7O57_RS21850 4451731..4452195 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F7O57_RS21855 4452250..4453059 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
F7O57_RS21860 4453308..4454588 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
F7O57_RS21865 4454611..4455084 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
F7O57_RS21870 4455095..4455874 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F7O57_RS21875 4455864..4456742 + 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F7O57_RS21880 4456760..4457194 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4441871..4452195 10324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T140859 WP_000347272.1 NZ_CP044403:4451731-4452195 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T140859 NZ_CP059488:51570-51821 [Acidithiobacillus ferrivorans]
ATGGATGCGATTACCTATACCACCGTTCGTGCAAACCTCGCCAGCGCCATGGACCGCGTTTGCGACGACCACGAACCCTT
GATCATTACCCGCAATGGCGAACAGTCCGTCGTGATGCTCTCGCTCGAAGACTACAAGGCGCTGGAGGAAACCGCCTACC
TCTTGCGTACCCCCGCCAACGCCAAGCGCCTTCTCGCGGCCGTCGCACAACTGAACGCCGGTAAAGGCGTTGAGCGGAAG
CTGGCGAAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140859 WP_001307405.1 NZ_CP044403:4451396-4451731 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140859 NZ_CP059488:51818-52072 [Acidithiobacillus ferrivorans]
GTGAGGCTGATCTTCTCTGACGATGCATGGGAAGACTACCTATATTGGCAGAAGCAAGACAAGCGCATGGTCGAGCGAAT
CAATAAACTGATACATGAGACACAGCGTGAACCCTTCGCGGGAGTTGGCAAACCCGAGGCACTGAAGCATGCTCTTTCAG
GATTTTGGTCACGCCGTATTACGGATGAGCATCGCATGGTGTATCGAATAGAAGGTGATGCATTGATGATTGCTCAGTTA
CGTTTCCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References