Detailed information of TA system TA140854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3973185..3973912
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: F7O57_RS19535
• Protein ID: WP_001521139.1
• Coordinates: 3973601..3973912 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7O57_RS19530
• Protein ID: WP_000126294.1
• Coordinates: 3973185..3973604 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS19510	3968270..3968797	-	528	WP_001078777.1	electron transport protein HydN	-
F7O57_RS19515	3968946..3969956	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
F7O57_RS19520	3970216..3971673	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
F7O57_RS19525	3971682..3973106	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
F7O57_RS19530	3973185..3973604	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
F7O57_RS19535	3973601..3973912	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
F7O57_RS19540	3974079..3974549	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
F7O57_RS19545	3974542..3974952	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
F7O57_RS19550	3974949..3975716	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
F7O57_RS19555	3975716..3976258	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
F7O57_RS19560	3976268..3977977	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3951026..4003510	52484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T140854 WP_001521139.1 NZ_CP044403:c3973912-3973601 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T140854 NZ_CP059481:c4360549-4360208 [Enterobacter kobei]
ATGAAAACTCTTCCTGCAAATCAGCGGGTGGCGAAACCATGCCCGCCGCTAGTACTTGTCTGGCAAACGTTGCTTACGCG
CCTACTGGAGCAACACTATGGCCTGACTCTGAATGACACGCCATTCTGCGATGAAACAGTCATTCAGGAACATATTAACG
CCGGTATCACACTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGCTCGTATCGATCGCAGGGGATTTAGC
TGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGACATTTTGCGGGCGCGGCAGGCAACGGGTCTTCTGTTACGACAAGA
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT140854 WP_000126294.1 NZ_CP044403:c3973604-3973185 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT140854 NZ_CP059481:c4360924-4360574 [Enterobacter kobei]
ATGCCGAATAACACACCGCCAGTAAACCATGACATCACCAAACCGTGGTGGGGGCTAAAACGCAGTATCACTCCGTGCTT
TGGCGCACGCTTGGTGCAGGAGGGCAACCGGCTGCACTATCTCGCCGACCGCGCCAGTATTATCGGGCAGTTCAGCGATG
CGGATTTACGTCATCTGGACCAGGCATTTCCTGTACTGATGAAACAGCTTGAACTGATGCTCACCAGCGGTGAGCTCAAT
CCACGGCACCAGCACTGCGTTACGCTGTACCACAACGAACTGACCTGTGAAGCGGATACGCTTGGTAGCTGTGGCTACGT
TTATCTCGCTTTCTATCCCACCCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9