Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3138100..3138602 Replicon chromosome
Accession NZ_CP044403
Organism Escherichia coli strain NMBU-W10C18

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag F7O57_RS15535 Protein ID WP_000767829.1
Coordinates 3138100..3138354 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag F7O57_RS15540 Protein ID WP_001259255.1
Coordinates 3138351..3138602 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7O57_RS15505 3133115..3133342 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
F7O57_RS15510 3133356..3134414 - 1059 WP_000492324.1 transport protein YeeE -
F7O57_RS15515 3134593..3135951 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
F7O57_RS15520 3135941..3136003 - 63 WP_010723108.1 membrane protein YoeI -
F7O57_RS15525 3136218..3137147 - 930 WP_023154044.1 LysR family transcriptional regulator -
F7O57_RS15530 3137193..3138017 - 825 WP_000754758.1 SDR family oxidoreductase -
F7O57_RS15535 3138100..3138354 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
F7O57_RS15540 3138351..3138602 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
F7O57_RS15545 3138885..3138935 + 51 WP_001364200.1 his operon leader peptide -
F7O57_RS15550 3139081..3139980 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
F7O57_RS15555 3139986..3141290 + 1305 WP_021523144.1 histidinol dehydrogenase -
F7O57_RS15560 3141287..3142357 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
F7O57_RS15565 3142357..3143424 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T140853 WP_000767829.1 NZ_CP044403:c3138354-3138100 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T140853 NZ_CP059481:3791441-3791659 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT140853 WP_001259255.1 NZ_CP044403:c3138602-3138351 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT140853 NZ_CP059481:3791039-3791413 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGTTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATCGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References