Detailed information of TA system TA140838

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2362174..2362545
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: F7O57_RS11635
• Protein ID: WP_001443846.1
• Coordinates: 2362396..2362545 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2362174..2362352 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS11610	2358129..2359502	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
F7O57_RS11615	2359631..2360566	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F7O57_RS11620	2360618..2361853	-	1236	WP_000040839.1	site-specific integrase	-
F7O57_RS11625	2361855..2362070	-	216	WP_000079604.1	excisionase XisR	-
F7O57_RS11630	2362170..2362358	-	189	WP_001302840.1	DUF1187 family protein	-
-	2362174..2362352	+	179	NuclAT_0	-	Antitoxin
-	2362174..2362352	+	179	NuclAT_0	-	Antitoxin
-	2362174..2362352	+	179	NuclAT_0	-	Antitoxin
-	2362174..2362352	+	179	NuclAT_0	-	Antitoxin
F7O57_RS11635	2362396..2362545	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F7O57_RS11640	2362601..2363410	-	810	WP_000166313.1	recombination protein RecT	-
F7O57_RS11645	2363403..2366003	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
F7O57_RS11650	2366105..2366380	-	276	WP_001344816.1	hypothetical protein	-
F7O57_RS11655	2366455..2366625	-	171	WP_001352098.1	conserved protein, Rac prophage	-
F7O57_RS11660	2366625..2366846	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2355805..2411047	55242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T140838 WP_001443846.1 NZ_CP044403:c2362545-2362396 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T140838 NZ_CP059478:9541-9828 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT140838 NZ_CP044403:2362174-2362352 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin