Detailed information of TA system TA140831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1117490..1118184
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: F7O57_RS05605
• Protein ID: WP_001521903.1
• Coordinates: 1117786..1118184 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: F7O57_RS05600
• Protein ID: WP_000554758.1
• Coordinates: 1117490..1117783 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS05575	1112605..1113354	+	750	WP_016233951.1	C40 family peptidase	-
F7O57_RS05580	1113411..1113764	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
F7O57_RS05585	1113843..1115582	-	1740	WP_021523009.1	flagellar biosynthesis protein FlhA	-
F7O57_RS05590	1115527..1116312	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F7O57_RS05595	1116383..1117438	+	1056	WP_001226177.1	DNA polymerase IV	-
F7O57_RS05600	1117490..1117783	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F7O57_RS05605	1117786..1118184	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F7O57_RS05610	1118194..1118646	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
F7O57_RS05615	1118891..1119145	+	255	WP_001521905.1	hypothetical protein	-
F7O57_RS05620	1119305..1119445	+	141	WP_166739706.1	peptide chain release factor	-
F7O57_RS05625	1119502..1120959	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F7O57_RS05630	1121220..1121678	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
F7O57_RS05635	1121770..1123014	+	1245	WP_000189541.1	esterase FrsA	-
-	1122274..1122354	+	81	NuclAT_11	-	-
-	1122274..1122354	+	81	NuclAT_11	-	-
-	1122274..1122354	+	81	NuclAT_11	-	-
-	1122274..1122354	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T140831 WP_001521903.1 NZ_CP044403:1117786-1118184 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140831 NZ_CP059469:23293-23577 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT140831 WP_000554758.1 NZ_CP044403:1117490-1117783 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140831 NZ_CP059469:23055-23303 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3