Detailed information of TA system TA140824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 153536..154138
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F7O57_RS01060
• Protein ID: WP_000897305.1
• Coordinates: 153536..153847 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7O57_RS01065
• Protein ID: WP_000356395.1
• Coordinates: 153848..154138 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS01040	148577..149014	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F7O57_RS01045	149059..150000	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F7O57_RS01050	150416..152230	+	1815	WP_020233236.1	hypothetical protein	-
F7O57_RS01055	152399..153307	-	909	WP_001306651.1	alpha/beta hydrolase	-
F7O57_RS01060	153536..153847	+	312	WP_000897305.1	hypothetical protein	Toxin
F7O57_RS01065	153848..154138	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
F7O57_RS01070	154223..154435	+	213	WP_000197769.1	hypothetical protein	-
F7O57_RS01075	154497..154775	+	279	WP_001306650.1	hypothetical protein	-
F7O57_RS01080	155194..155412	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
F7O57_RS01085	155631..155873	+	243	WP_021523315.1	hypothetical protein	-
F7O57_RS01090	156055..156984	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F7O57_RS01095	156981..157616	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
F7O57_RS01100	157613..158515	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T140824 WP_000897305.1 NZ_CP044403:153536-153847 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T140824 NZ_CP059468:1241618-1241902 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT140824 WP_000356395.1 NZ_CP044403:153848-154138 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT140824 NZ_CP059468:1241386-1241628 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7